Bovine Fibronectin,FN ELISA Kit

Code CSB-E13781B
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
fibronectin 1
Alternative Names
FN1Fibronectin ELISA Kit; FN) [Cleaved into: Anastellin] ELISA Kit
Abbreviation
FN1
Uniprot No.
Species
Bos taurus (Bovine)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates, cell lysates
Detection Range
0.137 μg/mL-100 μg/mL
Sensitivity
0.137 μg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine FN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:5 Average % 92    
Range % 85-102    
1:10 Average % 97    
Range % 91-110    
1:20 Average % 89    
Range % 83-96    
1:40 Average % 95    
Range % 84-100    
Recovery
The recovery of bovine FN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 96 88-105    
EDTA plasma (n=4) 97 92-105    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
µg/ml OD1 OD2 Average Corrected    
100 1.839 1.803 1.821 1.677    
33 1.657 1.649 1.653 1.509    
11 1.451 1.435 1.443 1.299    
3.7 0.958 0.992 0.975 0.831    
1.2 0.643 0.667 0.655 0.511    
0.4 0.372 0.354 0.363 0.219    
0.13 0.251 0.243 0.247 0.103    
0 0.145 0.142 0.144      
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Bovine FN1 ELISA Kit was designed for the quantitative measurement of Bovine FN1 protein in serum, plasma, cell culture supernates, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.137 μg/mL-100 μg/mL and the sensitivity is 0.137 μg/mL.

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Target Background

Function
(From Uniprot)
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.; Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
Subcellular Location
Secreted, extracellular space, extracellular matrix.
Tissue Specificity
Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix.
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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