Bovine Inhibin beta A chain(INHBA) ELISA kit

Code CSB-EL011719BO
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
inhibin, beta A
Alternative Names
INHBAInhibin beta A chain ELISA Kit; Activin beta-A chain ELISA Kit
Abbreviation
INHBA
Uniprot No.
Species
Bos taurus (Bovine)
Sample Types
serum, plasma, tissue homogenates
Detection Range
15 pg/mL-600 pg/mL
Sensitivity
15 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine INHBA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 95  
Range % 91-98  
1:2 Average % 94  
Range % 86-103  
1:4 Average % 92  
Range % 89-98  
1:8 Average % 96  
Range % 91-102  
Recovery
The recovery of bovine INHBA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 92-100  
EDTA plasma (n=4) 96 91-103  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
600 0.161 0.159 0.160    
300 0.371 0.353 0.362    
138 0.586 0.555 0.571    
45 0.867 0.874 0.871    
15 1.489 1.492 1.491    
0 2.368 2.441 2.405    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Bovine INHBA ELISA Kit was designed for the quantitative measurement of Bovine INHBA protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 15 pg/mL-600 pg/mL and the sensitivity is 15 pg/mL.

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Target Background

Function
(From Uniprot)
Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.
Gene References into Functions
  1. Results confirm the prodevelopmental actions of activin A and indicate that CTGF may also function as an embryokine by regulating the number of ICM cells in the blastocyst and altering gene expression. Low concentrations of HGF were inhibitory to development. PMID: 27185102
  2. The expression of the components of the activin-inhibin-follistatin system is altered in bovine cystic ovarian disease. PMID: 25001504
  3. Results support a physiological role for granulosa-derived inhibin as an antagonist of bone morphogenetic protein action on thecal androgen synthesis. PMID: 20739376
  4. activin AB is a lignd for activin type IB receptor ALK7 and is responsible for activin-mediated secretion of insulin from pancreatic beta cell line, MIN6. PMID: 15196700
Subcellular Location
Secreted.
Protein Families
TGF-beta family
Database Links
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