Code | CSB-E08893b |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<15% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of bovine IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 90 | ||||
Range % | 86-94 | |||||
1:2 | Average % | 96 | ||||
Range % | 92-100 | |||||
1:4 | Average % | 83 | ||||
Range % | 80-86 | |||||
1:8 | Average % | 91 | ||||
Range % | 83-98 |
The recovery of bovine IGF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 94 | 87-99 | ||||
EDTA plasma (n=4) | 93 | 91-96 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||
|
This Bovine IGF1 ELISA Kit was designed for the quantitative measurement of Bovine IGF1 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.75 ng/mL-60 ng/mL and the sensitivity is 0.94 ng/mL.
There are currently no reviews for this product.
Does the Bovine Insulin-like growth factor 1, IGF-1 ELISA Kit (CSB-E08893b) assay measure total bovine IGF-1 or only free IGF-1? I notice there is no acidification step to dissociate binding proteins, which is why I ask.
This Bovine Insulin-like growth factor 1, IGF-1 ELISA Kit is for the detection of total IGF-1.