Bovine Interferon α,IFN-α ELISA Kit

Code CSB-E13726B
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Alternative Names
Interferon alpha-1 ELISA Kit
Abbreviation
IFN-α
Uniprot No.
Species
Bos taurus (Bovine)
Sample Types
serum, plasma
Detection Range
25 pg/mL-1000 pg/mL
Sensitivity
25 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine IFN-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 96  
Range % 91-101  
1:2 Average % 91  
Range % 85-98  
1:4 Average % 95  
Range % 90-103  
1:8 Average % 96  
Range % 90-102  
Recovery
The recovery of bovine IFN-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 89-98  
EDTA plasma (n=4) 97 91-103  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
1000 0.156 0.149 0.153    
500 0.362 0.336 0.349    
230 0.561 0.541 0.551    
75 0.865 0.856 0.861    
25 1.542 1.508 1.525    
0 2.390 2.356 2.373    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Bovine IFN-α ELISA Kit was designed for the quantitative measurement of Bovine IFN-α protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 25 pg/mL-1000 pg/mL and the sensitivity is 25 pg/mL.

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Target Background

Function
(From Uniprot)
Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
Subcellular Location
Secreted.
Protein Families
Alpha/beta interferon family
Database Links
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