Bovine Interleukin 1,IL-1 ELISA Kit

Bovine Interleukin 1,IL-1 ELISA Kit

Product Details

Citations

Customer Reviews and Q&A

Target Background

Product Details

Citations

Related Products

Customer Reviews and Q&A

Target Background

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ELISA Kit
Bovine Interleukin 1,IL-1 ELISA Kit

Citations (9) Protocol

Code	CSB-E12019B
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details
Citations (9)
Related Products
Customer Reviews and Q&A
Target Background

Target Name

Interleukin 1,IL-1

Alternative Names

IL1A ELISA Kit; Interleukin-1 alpha ELISA Kit; IL-1 alpha ELISA Kit

Abbreviation

IL1A

Uniprot No.

P08831

Species

Bos taurus (Bovine)

Sample Types

serum, plasma, tissue homogenates

Detection Range

250 pg/mL-4000 pg/mL

Sensitivity

250 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-94
1:2	Average %	92
	Range %	87-97
1:4	Average %	84
	Range %	80-88
1:8	Average %	98
	Range %	90-102

Recovery

The recovery of bovine IL-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	89	83-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
4000	0.147	0.141	0.144
2000	0.296	0.279	0.288
1000	0.431	0.467	0.449
500	0.782	0.823	0.803
250	1.862	1.826	1.844

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Bovine IL1A ELISA Kit was designed for the quantitative measurement of Bovine IL1A protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 250 pg/mL-4000 pg/mL and the sensitivity is 250 pg/mL.

Effect of non-encapsulated and encapsulated active dried yeast on blood cell count, blood metabolites and immune response of finishing beef heifers P Jiao,Canadian Journal of Animal Science ja,2021
Milk fever in dairy cows is preceded by activation of innate immunity and alterations in carbohydrate metabolism prior to disease occurrence Zhang G.et al,Res Vet Sci,2017
Dairy cows affected by ketosis show alterations in innate immunity and lipid and carbohydrate metabolism during the dry off period and postpartum Zhang G.et al,Research in Veterinary Science,2016
Detection of serum amyloid-A concentration in the calf clinically diagnosed with pneumonia, enteritis and pneumoenteritis Mustafa Kabu. et al,/,2016
Occurrence of retained placenta is preceded by an inflammatory state and alterations of energy metabolism in transition dairy cows. Dervishi E.et al,J Anim Sci Biotechnol.,2016
Determining Serum Haptoglobin and Cytokine Concentrations in Diarrheic Calves ALBAYRAK H,.et al,/,2016
Nrf2-ARE signals mediated the anti-oxidative action of electro-acupuncture in an MPTP mouse model of Parkinson´s disease Lv E. et al,Free Radic Res.,2015
Innate immunity and carbohydrate metabolism alterations precede occurrence of subclinical mastitis in transition dairy cows. Dervishi E.et al,J Anim Sci Technol.,2015
Oral administration of LPS and lipoteichoic acid prepartum modulated reactants of innate and humoral immunity in periparturient dairy cows Iqbal S et al,Innate Immun,2013

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Function
(From Uniprot)

Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.

Gene References into Functions

These findings suggest that the elevated levels of IL-1alpha found in the osteoarthritis environment heighten fibroblast-like synoviocytes sensitivity to fluid shear by altering both intercellular communication and individual cell sensitivity, which could affect downstream functions and contribute to progression of the disease state PMID: 28716465
Protein kinase R plays a pivotal role in oncostatin M and interleukin-1 signalling in bovine articular cartilage chondrocytes. PMID: 22287113
Testicular IL-1 alpha and IL-1 beta concentrations were highest in the early post-natal period; however, IL-1 bioactivity and IL-6 concentrations were greatest in the immediate pre-pubertal period. PMID: 19144032
Genes for IL-1alpha and IL-1beta are expressed and a functional IL-1R is present in bovine corpora lutea throughout luteal phase. IL-1alpha and IL-1beta may have different roles as regulating PGF(2alpha) and PGE(2) production during luteal phase. PMID: 15253926
non-metalloproteinase mechanisms participate in IL-1-induced matrix degradation and loss of tissue material properties PMID: 17174540
These results suggest that IL-1alpha and IL-1beta are produced by the stromal cells, that IL-1beta is produced by the epithelial cells, and that IL-1alpha is a more potent stimulator of prostaglandin and plasminogen activator in bovine endometrial cells. PMID: 18930361

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Subcellular Location

Cytoplasm. Secreted.

Protein Families

IL-1 family

Database Links

KEGG: bta:281250

STRING: 9913.ENSBTAP00000013665

UniGene: Bt.191

Promotions

Transmembrane Protein Questionnaire with Gifts

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

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Related pathways

MAPK signaling pathway Necroptosis Cellular senescence Hematopoietic cell lineage Osteoclast differentiation AGE-RAGE signaling pathway in diabetic complications

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.