Bovine Lysozyme (LZM) ELISA Kit

Code CSB-E15829B
Size 96T,5×96T,10×96T
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Product Details

Target Name
Lysozyme (LZM)
Alternative Names
LYZ1Lysozyme C ELISA Kit; EC 3.2.1.17 ELISA Kit; 1,4-beta-N-acetylmuramidase C ELISA Kit
Abbreviation
LZM
Uniprot No.
Species
Bos taurus (Bovine)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.312 ng/mL-20 ng/mL
Sensitivity
0.156 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine LZM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:200 Average % 101
Range % 96-105
1:400 Average % 90
Range % 85-95
1:800 Average % 106
Range % 100-110
1:1600 Average % 104
Range % 99-109
Recovery
The recovery of bovine LZM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 94 87-98
EDTA plasma (n=4) 100 94-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average
20 0.147 0.143 0.145
10 0.187 0.195 0.191
5 0.286 0.273 0.280
2.5 0.404 0.399 0.402
1.25 0.634 0.604 0.619
0.625 0.970 0.934 0.952
0.312 1.809 1.822 1.816
0 2.516 2.457 2.487
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Bovine LZM ELISA Kit was designed for the quantitative measurement of Bovine LZM protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.156 ng/mL.

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Target Background

Function
(From Uniprot)
Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.
Protein Families
Glycosyl hydrolase 22 family
Tissue Specificity
Stomach-specific.
Database Links

KEGG: bta:280849

UniGene: Bt.29367

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