Bovine Parathyroid hormone(PTH) ELISA kit

Code CSB-EL018987BO
Size 96T,5×96T,10×96T
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Product Details

Target Name
parathyroid hormone
Alternative Names
PTH ELISA Kit; Parathyroid hormone ELISA Kit; PTH ELISA Kit; Parathyrin ELISA Kit
Abbreviation
PTH
Uniprot No.
Species
Bos taurus (Bovine)
Sample Types
serum, plasma, tissue homogenates
Detection Range
31.25 pg/mL-2000 pg/mL
Sensitivity
7.8 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine PTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 91
Range % 86-99
1:2 Average % 96
Range % 90-110
1:4 Average % 90
Range % 83-99
1:8 Average % 95
Range % 85-105
Recovery
The recovery of bovine PTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 92 88-98
EDTA plasma (n=4) 96 90-110
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2000 2.626 2.603 2.615 2.498
1000 2.365 2.325 2.345 2.228
500 1.986 1.992 1.989 1.872
250 1.502 1.486 1.494 1.377
125 0.841 0.835 0.838 0.721
62.5 0.486 0.474 0.480 0.363
31.25 0.371 0.375 0.373 0.256
0 0.115 0.119 0.117  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Bovine PTH ELISA Kit was designed for the quantitative measurement of Bovine PTH protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.

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Target Background

Function
(From Uniprot)
PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.
Gene References into Functions
  1. These data highlight the ability of PTH to phosphorylate beta-catenin directly via PKA. PMID: 26851123
  2. These data suggest that prostaglandin E2 acting via EP4R on bone marrow macrophages committed to the osteoblast cell lineage, stimulated secretion of a factor or factors that acted to suppress PTH-stimulated osteoblast differentiation. PMID: 23639875
  3. These results suggest that in vivo PTH treatment increased in vitro osteoclastogenesis and resorption without altering the number of osteoclast precursors. PMID: 21611963
  4. Osteoblast 2-deoxyglucose uptake and glycogen synthesis were increased after exposure to low concentrations (0.1 nmol/l and above) of PTH. PMID: 21076856
  5. Parathyroid hormone stimulated growth and decreased Col-X deposition via phosphotidylinositol-3,4,5 triphosphate kinase and mitogen activating protein kinase pathways in avian sterna. PMID: 19957341
  6. likely involvement of the Sp family in regulating PTH gene expression through interactions with an Sp1 DNA element in the hormone's promoter. PMID: 12810570
  7. alternative cis-acting protein-binding elements may determine the regulation of PTH mRNA stability in response to changes in serum calcium and phosphate PMID: 15824859
  8. a possible role for the Wnt signaling pathway in PTH actions in bone PMID: 15962290
  9. Data suggest that calcium-mediated destabilization of parathyroid hormone mRNA requires gene transcription and involves increases in cytosolic Ca. PMID: 18096993

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Subcellular Location
Secreted.
Protein Families
Parathyroid hormone family
Database Links

KEGG: bta:280903

STRING: 9913.ENSBTAP00000025399

UniGene: Bt.95

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