Bovine Plasminogen(PLG) ELISA kit

Code CSB-EL018188BO
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Alternative Names
PLG ELISA Kit; Plasminogen ELISA Kit; EC 3.4.21.7) [Cleaved into: Plasmin heavy chain A; Activation peptide; Plasmin heavy chain A ELISA Kit; short form; Plasmin light chain B] ELISA Kit
Abbreviation
PLG
Uniprot No.
Species
Bos taurus (Bovine)
Sample Types
serum, plasma, tissue homogenates
Detection Range
62.5 ng/mL-4000 ng/mL
Sensitivity
15.6 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine PLG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:100 Average % 97    
Range % 93-102    
1:200 Average % 85    
Range % 81-90    
1:400 Average % 102    
Range % 97-106    
1:800 Average % 91    
Range % 86-95    
Recovery
The recovery of bovine PLG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 93 87-96    
EDTA plasma (n=4) 105 98-109    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
ng/ml OD1 OD2 Average      
5000 0.102 0.099 0.101      
2500 0.167 0.160 0.164      
1250 0.254 0.241 0.248      
625 0.372 0.365 0.369      
312.5 0.558 0.544 0.551      
156.2 0.766 0.713 0.740      
78 1.024 0.984 1.004      
0 1.834 1.765 1.800      
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Bovine PLG ELISA Kit was designed for the quantitative measurement of Bovine PLG protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 62.5 ng/mL-4000 ng/mL and the sensitivity is 15.6 ng/mL.

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Target Background

Function
(From Uniprot)
Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells.
Gene References into Functions
  1. SUPA formed a higher avidity complex (118-fold) with bovine Pg than with human Pg and non-proteolytically activated bovine but not human Pg. PMID: 22518846
  2. The plasminogen/plasminogen activator/plasmin system is activated during gamete interaction and regulates the sperm entry into the oocyte. PMID: 22177313
  3. The current study suggests the existence of an active TNF-alpha-plasminogen-plasmin autocrine/paracrine loop on the massively infiltrated polymorphonuclear neutrophils inside udders of drying-off cows. PMID: 19638263
  4. examination of interaction with streptococcal plasminogen activator PauA PMID: 15351638
Subcellular Location
Secreted.
Protein Families
Peptidase S1 family, Plasminogen subfamily
Database Links

KEGG: bta:280897

STRING: 9913.ENSBTAP00000001674

UniGene: Bt.306

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