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Product Name:

Bovine Toll-like receptor 2(TLR2) ELISA kit

Product Type : ELISA Kit
Code : CSB-EL023601BO
Size : 96T,5×96T,10×96T
Uniprot NO. : Q95LA9
Abbreviation : TLR2
Protein Biological Process 1 : Immunity
Alias : CD282, TIL4, toll/interleukin 1 receptor-like 4
Species : Bovine
Protein Biological Process 3 : Immunity
Sample Types : serum, plasma, tissue homogenates
Detect Range : 15.6 ng/ml-1000 ng/ml
Sensitivity : 3.9ng/ml
Assay Time : 1-5h
Sample Volume : 50-100ul
Detection Wavelengt : 450 nm
Availability : 5-7 working days
Research Area : Immunology
Protocol :
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Target details : This protein is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB.
HGNC : 11848
RGD : 735138
MGI : 1346060
Precision :
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity :
To assess the linearity of the assay, samples were spiked with high concentrations of bovine TLR2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 95
Range % 90-100
1:2 Average % 93
Range % 89-97
1:4 Average % 84
Range % 80-88
1:8 Average % 98
Range % 94-102
Recovery :
The recovery of bovine TLR2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 106 102-114
EDTA plasma (n=4) 97 92-101
Typical Data :
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
1000 2.215 2.347 2.281 2.109
500 1.627 1.705 1.666 1.494
250 1.043 1.096 1.070 0.898
125 0.701 0.680 0.691 0.519
62.5 0.469 0.472 0.471 0.299
31.2 0.352 0.364 0.358 0.186
15.6 0.274 0.259 0.267 0.095
0 0.175 0.168 0.172  
Reference :

1. Modulation of Bovine Wnt Signaling Pathway Genes by Cowpea Phenolic Extract,Sarah Adjei-Fremah.et al,Agricultural Science,2016,8(3)

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