Canine Luteinizing Hormone,LH ELISA Kit

Code CSB-E06790c
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
Luteinizing Hormone,LH
Alternative Names
LHBLutropin subunit beta ELISA Kit; Luteinizing hormone subunit beta ELISA Kit; LH-B ELISA Kit; LSH-B ELISA Kit; LSH-beta ELISA Kit; Lutropin beta chain ELISA Kit; Fragment ELISA Kit
Abbreviation
LH
Uniprot No.
Species
Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.75 mIU/mL-60 mIU/mL
Sensitivity
0.94 mIU/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of canine LH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %94
Range %90-98
1:2Average %96
Range %92-99
1:4Average %101
Range %98-105
1:8Average %83
Range %80-87
Recovery
The recovery of canine LH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8684-92
EDTA plasma (n=4)9892-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mIU/mlOD1OD2Average
600.350 0.357 0.354
22.50.476 0.468 0.472
11.250.619 0.605 0.612
3.751.008 0.998 1.003
0.751.594 1.512 1.553
02.008 2.113 2.061
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Dog Luteinizing Hormone (LH) ELISA Kit was designed for the quantitative measurement of Dog Luteinizing Hormone (LH) protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.75 mIU/mL-60 mIU/mL and the sensitivity is 0.94 mIU/mL.

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Target Background

Function
(From Uniprot)
Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids.
Subcellular Location
Secreted.
Protein Families
Glycoprotein hormones subunit beta family
Database Links
CUSABIO guaranteed quality
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