Chicken Immunoglobulin of Yolk,IgY ELISA Kit

Code CSB-E11635Ch
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Abbreviation
IgY
Species
Gallus gallus (Chicken)
Sample Types
serum, plasma, egg yolk
Detection Range
0.078 ng/mL-5 ng/mL
Sensitivity
0.02 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of chicken IgY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:500000 Average % 89  
Range % 81-100  
1:1000000 Average % 96  
Range % 87-103  
1:2000000 Average % 97  
Range % 92-104  
1:4000000 Average % 92  
Range % 86-98  
Recovery
The recovery of chicken IgY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-105  
EDTA plasma (n=4) 97 90-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
5 2.781 2.911 2.846 2.737  
2.5 2.316 2.342 2.329 2.220  
1.25 1.579 1.584 1.582 1.473  
0.625 0.901 0.883 0.892 0.783  
0.312 0.443 0.436 0.440 0.331  
0.156 0.315 0.319 0.317 0.208  
0.078 0.249 0.247 0.248 0.139  
0 0.108 0.110 0.109    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

The Chicken Immunoglobulin of Yolk (IgY) ELISA Kit is a highly sensitive and reliable tool for detecting IgY in chicken serum, plasma, and egg yolk samples. Developed using cutting-edge technology, this ELISA kit delivers accurate results with a detection range of 0.078 ng/mL-5 ng/mL and a sensitivity of 0.02 ng/mL.

This kit offers a quick and convenient assay time of 1-5 hours, with a sample volume of 50-100ul. The detection wavelength is 450 nm, making it ideal for immunology research applications. The assay principle is quantitative and uses a sandwich method of measurement, ensuring accurate and precise detection of IgY.

The Chicken Immunoglobulin of Yolk (IgY) ELISA Kit has been extensively tested, with more than 4 citations attesting to its effectiveness and reliability. It is specifically designed for researchers and scientists who demand high-quality and reproducible results. This ELISA kit is a good choice for those studying the immune system of chickens and conducting immunology research. It offers high accuracy, sensitivity, and convenience, making it an indispensable tool for any laboratory.

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