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Product Name:

Dog Interleukin-1 alpha(IL1A) ELISA kit

Product Type : ELISA Kit
Code : CSB-EL011613DO
Size : 96T,5×96T,10×96T
Uniprot NO. : O46612
Abbreviation : IL1A
Protein Biological Process 1 : Immunity
Alias : IL-1A, IL1, IL1-ALPHA, IL1F1, IL1A (IL1F1)|OTTHUMP00000203692|hematopoietin-1|preinterleukin 1 alpha|pro-interleukin-1-alpha
Species : Dog
Protein Biological Process 3 : Inflammatory response
Sample Types : serum, plasma, cell culture supernates, tissue homogenates
Detect Range : 9.38 pg/ml-600 pg/ml
Sensitivity : 2.34pg/ml
Assay Time : 1-5h
Sample Volume : 50-100ul
Detection Wavelengt : 450 nm
Availability : 5-7 working days
Research Area : Immunology
Protocol :
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Target details : This protein is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer s disease.
HGNC : 5991
RGD : 2890
MGI : 96542
Precision :
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity :
To assess the linearity of the assay, samples were spiked with high concentrations of dog IL1A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 85
Range % 81-89
1:2 Average % 102
Range % 97-107
1:4 Average % 89
Range % 84-93
1:8 Average % 105
Range % 101-109
Recovery :
The recovery of dog IL1A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 87 83-90
EDTA plasma (n=4) 97 90-100
Typical Data :
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
600 3.029 3.000 3.015 2.919
300 2.506 2.467 2.487 2.391
150 1.731 1.768 1.750 1.654
75 1.015 1.065 1.040 0.944
37.5 0.496 0.488 0.492 0.396
18.75 0.314 0.305 0.310 0.214
9.38 0.201 0.198 0.200 0.104
0 0.098 0.094 0.096  
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