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Product Name:

Dog growth hormone,GH ELISA Kit

Product Type : ELISA Kit
Code : CSB-E14962c
Size : 96T,5×96T,10×96T
Uniprot NO. : P33711
Abbreviation : GH1
Alias : GH, GH-N, GHN, IGHD1B, hGH-N, pituitary growth hormone
Species : Dog
Sample Types : serum, plasma and tissue homogenates
Detect Range : 12.5 ng/ml-200 ng/ml
Sensitivity : 2.5ng/ml
Assay Time : 1-5h
Sample Volume : 50-100ul
Detection Wavelengt : 450 nm
Availability : 3-5 working days
Protocol :
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Target details : This protein is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus. Mutations in or deletions of the gene lead to growth hormone deficiency and short stature.
Precision :
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity :
To assess the linearity of the assay, samples were spiked with high concentrations of dog GH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %92
Range %88-99
1:2Average %96
Range %91-102
1:4Average %97
Range %90-105
1:8Average %95
Range %91-99
Recovery :
The recovery of dog GH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9892-103
EDTA plasma (n=4)9589-100
Typical Data :
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
2000.147 0.148 0.148
1000.295 0.304 0.300
500.562 0.565 0.564
251.281 1.278 1.280
12.52.322 2.325 2.324
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