Fish Acetylcholinesterase(AChE)ELISA Kit

Code CSB-E17001Fh
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
Acetylcholinesterase(AChE)
Alternative Names
acheAcetylcholinesterase ELISA kit; AChE ELISA kit; EC 3.1.1.7 ELISA kit
Abbreviation
AChE
Uniprot No.
Species
Fish
Sample Types
serum, plasma, tissue homogenates
Detection Range
2.5 ng/mL-40 ng/mL
Sensitivity
2.5 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Neuroscience
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of fish AChE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 94
Range % 89-103
1:2 Average % 92
Range % 87-101
1:4 Average % 93
Range % 87-100
1:8 Average % 89
Range % 83-99
Recovery
The recovery of fish AChE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 89 84-93
EDTA plasma (n=4) 94 87-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average  
40 0.284 0.292 0.288  
20 0.521 0.532 0.527  
10 0.886 0.891 0.889  
5 1.374 1.382 1.378  
2.5 2.583 2.599 2.591  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Fish AChE ELISA Kit was designed for the quantitative measurement of Fish AChE protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 2.5 ng/mL-40 ng/mL and the sensitivity is 2.5 ng/mL.

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Target Background

Function
(From Uniprot)
Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. May be involved in cell-cell interactions.
Subcellular Location
[Isoform H]: Cell membrane; Lipid-anchor, GPI-anchor. Cell junction, synapse.; [Isoform T]: Cell membrane; Peripheral membrane protein. Cell junction, synapse. Note=Attached to the membrane through disulfide linkage with the collagenic subunit, itself bound to the membrane.
Protein Families
Type-B carboxylesterase/lipase family
Tissue Specificity
Found in the synapses and to a lower extent in extrajunctional areas of muscle and nerve, and on erythrocyte membranes.
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