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| Code | CSB-E09861h |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of human 2, 3-DPG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 92-100 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 92-103 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-99 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 85-96 | |||||
| The recovery of human 2, 3-DPG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 85-94 | ||||
| EDTA plasma (n=4) | 84 | 80-89 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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This Human 2,3DPG ELISA Kit was designed for the quantitative measurement of Human 2,3DPG protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.312 μmol/mL-20 μmol/mL and the sensitivity is 0.078 μmol/mL.
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Could you please advise me the below:
“you had any data to support using this kit for samples from Red Blood Cell units? These blood units typically are collected/stored with a citrate anticoagulant. We currently use a deproteinzation procedure to extract the DPG from the cells.”
Any/all feedback you might have would be greatly appreciated!
I am changing my method for the detection of 2,3-DPG for red blood cell concentrates, and I am interested in trying your ELISA Kit CSB-E09861h. Do you have a recommendation for centrifugation parameters prior to using the assay, if I would want to analyze an ordinary EDTA sample? Rcf, time, temperature...? -force (rcf), single- or double centrifugation cycle, time of the cycle(s), and temperature during centrifugation.
No special requirements, please directly refer to our RBC sample processing method(as below) for testing.
For the selection of anticoagulants, we generally use EDTA, the specific methods and requirements can be found in the sample preparation method as below.We have not tested the red blood cell sample, so it is recommended that you do a pre-experiment.Cell Lysates (1) Adherent Cell: Remove media and rinse cells once with ice-cold PBS (pH 7.2-7.4). Scrape cells off the plate and transfer them to an appropriate tube. Dilute cell suspension with 1xPBS (pH 7.2-7.4), until cell concentration reached 100 million/ml. Then store overnight at -20°C. After two freeze-thaw cycles to break up the cell membranes, the cell lysates were centrifuged for 5 minutes at 5000 x g, 2 - 8°C. Collect the supernatant. Cell lysates should be assayed immediately or aliquotted and stored at -20°C. Centrifuge the sample again after thawing before the assay. Avoid repeated freeze-thaw cycles. (2) Suspension Cell: Collect cells with an appropriate tube, and centrifuge for 5 minutes at 1000x g, 2 - 8°C. Remove the supernatant and resuspend cells with 1xPBS (pH 7.2-7.4). Centrifuge for 5 minutes at 1000 x g, 2 - 8°C. Remove the supernatant. Dilute the cell with 1xPBS (pH 7.2-7.4), until cell concentration reached 100 million/ml. Then store overnight at -20°C. After two freeze-thaw cycles to break up the cell membranes, the cell lysates were centrifuged for 5 minutes at 5000 x g, 2 - 8°C. Collect the supernatant. Cell lysates should be assayed immediately or aliquotted and stored at -20°C. Centrifuge the sample again after thawing before the assay. Avoid repeated freeze-thaw cycles.
