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| Code | CSB-E08363h |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of human 5-HT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 92-102 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 91-98 | |||||
| 1:4 | Average % | 85 | ||||
| Range % | 80-89 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 89-99 | |||||
| The recovery of human 5-HT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-99 | ||||
| EDTA plasma (n=4) | 89 | 85-96 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||
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This Human 5HT ELISA Kit was designed for the quantitative measurement of Human 5HT protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 4 ng/mL-800 ng/mL and the sensitivity is 2 ng/mL.
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