Human Alpha-N-acetylgalactosaminidase(NAGA) ELISA kit

Code CSB-EL015414HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
N-acetylgalactosaminidase, alpha-
Alternative Names
NAGA ELISA Kit; Alpha-N-acetylgalactosaminidase ELISA Kit; EC 3.2.1.49 ELISA Kit; Alpha-galactosidase B ELISA Kit
Abbreviation
NAGA
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, urine, saliva, tissue homogenates
Detection Range
0.625 ng/mL-40 ng/mL
Sensitivity
0.156 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human NAGA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %94
Range %90-98
1:2Average %97
Range %92-101
1:4Average %86
Range %82-90
1:8Average %105
Range %100-109
Recovery
The recovery of human NAGA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9589-98
EDTA plasma (n=4)9288-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
402.246 2.152 2.199 1.980
201.892 1.815 1.854 1.635
101.465 1.504 1.485 1.266
51.127 1.179 1.153 0.934
2.50.874 0.836 0.855 0.636
1.250.541 0.568 0.555 0.336
0.6250.362 0.349 0.356 0.137
00.221 0.217 0.219
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human NAGA ELISA Kit was designed for the quantitative measurement of Human NAGA protein in serum, plasma, urine, saliva, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

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Target Background

Function
(From Uniprot)
Removes terminal alpha-N-acetylgalactosamine residues from glycolipids and glycopeptides. Required for the breakdown of glycolipids.
Gene References into Functions
  1. results demonstrate the utility of eQTL mapping in the identification of novel asthma genes and provide evidence for the importance of FADS2, NAGA, and F13A1 in the pathogenesis of asthma. PMID: 24934276
  2. the active sites of human lysosomal enzymes alpha-galactosidase and alpha-N-acetylgalactosaminidase have interconvertible specificites PMID: 20444686
  3. Tumor cell alpha-N-acetylgalactosaminidase activity and its involvement in GcMAF-related macrophage activation. PMID: 12062184
  4. Structural analysis revealed biochemical and phenotypic differences in these Kanzaki patients with the R329Q and R329W mutation. PMID: 15619430
  5. NAGA mutation p.D217N (c.649G>A) in exon 6 and mutation p.E325K (c.973G>A) in exon 8 may have roles in alpha-N-acetylgalactosaminidase deficiency with cardiomyopathy PMID: 17171432
  6. Specific enzymatic activity of serum alpha-N-acetylgalactosaminidase was significantly increased in stage III melanoma patients, but not in early stages. PMID: 19394758
  7. The active site of human alpha-NAGAL has anomeric selectivity for its catalytic product and the structure reveals a novel active-site rearrangement upon hexose ligand binding. PMID: 19683538
  8. Use of a modified NAGA in the development of enzyme replacement therapy for Fabry disease is reported. PMID: 19853240

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Involvement in disease
Schindler disease (SCHIND); Kanzaki disease (KANZD)
Subcellular Location
Lysosome.
Protein Families
Glycosyl hydrolase 27 family
Database Links

HGNC: 7631

OMIM: 104170

KEGG: hsa:4668

STRING: 9606.ENSP00000379680

UniGene: Hs.75372

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