Human Centromere protein K(CENPK) ELISA kit

Code CSB-EL005214HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
centromere protein K
Alternative Names
CENPK ELISA Kit; ICEN37 ELISA Kit; FKSG14 ELISA Kit; Centromere protein K ELISA Kit; CENP-K ELISA Kit; Interphase centromere complex protein 37 ELISA Kit; Protein AF-5alpha ELISA Kit; p33 ELISA Kit
Abbreviation
CENPK
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
23.5 pg/mL-1500 pg/mL
Sensitivity
5.8 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CENPK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %93
Range %88-97
1:2Average %100
Range %90-105
1:4Average %92
Range %85-96
1:8Average %92
Range %84-96
Recovery
The recovery of human CENPK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9185-95
EDTA plasma (n=4)9990-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
15001.889 1.966 1.928 1.798
7501.426 1.476 1.451 1.321
3750.921 0.900 0.911 0.781
187.50.566 0.543 0.555 0.425
940.369 0.345 0.357 0.227
470.259 0.263 0.261 0.131
23.50.199 0.192 0.196 0.066
00.131 0.129 0.130
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human CENPK ELISA Kit was designed for the quantitative measurement of Human CENPK protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.8 pg/mL.

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Target Background

Function
(From Uniprot)
Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Acts in coordination with KNL1 to recruit the NDC80 complex to the outer kinetochore.
Involvement in disease
Chromosomal aberrations involving CENPK are a cause of acute leukemias. Translocation t(5;11)(q12;q23) with KMT2A/MLL1.
Subcellular Location
Nucleus. Chromosome, centromere. Chromosome, centromere, kinetochore. Note=Localizes exclusively in the centromeres. The CENPA-CAD complex is probably recruited on centromeres by the CENPA-NAC complex.
Protein Families
CENP-K/MCM22 family
Tissue Specificity
Detected in several fetal organs with highest levels in fetal liver. In adults, it is weakly expressed in lung and placenta.
Database Links

HGNC: 29479

OMIM: 611502

KEGG: hsa:64105

STRING: 9606.ENSP00000242872

UniGene: Hs.529778

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