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Human Coagulation factor XIII B chain(F13B) ELISA kit

Citations (1) Protocol
Code CSB-EL007922HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
coagulation factor XIII, B polypeptide
Alternative Names
F13B ELISA Kit; Coagulation factor XIII B chain ELISA Kit; Fibrin-stabilizing factor B subunit ELISA Kit; Protein-glutamine gamma-glutamyltransferase B chain ELISA Kit; Transglutaminase B chain ELISA Kit
Abbreviation
F13B
Uniprot No.
P05160
Species
Homo sapiens (Human)
Sample Types
serum, plasma, urine, tissue homogenates
Detection Range
0.312 ng/mL-20 ng/mL
Sensitivity
0.078 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Blood Coagulation
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human F13B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)
1:100 Average % 85
Range % 81-89
1:200 Average % 93
Range % 90-96
1:400 Average % 104
Range % 100-108
1:800 Average % 99
Range % 95-103
Recovery
The recovery of human F13B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 107 103-110
EDTA plasma (n=4) 89 86-91
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
20 2.734 2.654 2.694 2.617
10 2.122 2.205 2.164 2.087
5 1.497 1.487 1.492 1.415
2.5 0.875 0.898 0.887 0.810
1.25 0.533 0.568 0.551 0.474
0.625 0.331 0.352 0.342 0.265
0.312 0.206 0.198 0.202 0.125
0 0.079 0.075 0.077 　
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human F13B ELISA Kit was designed for the quantitative measurement of Human F13B protein in serum, plasma, urine, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

Citations

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F13B antibodies

F13B Proteins

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Target Background

Function
(From Uniprot)
The B chain of factor XIII is not catalytically active, but is thought to stabilize the A subunits and regulate the rate of transglutaminase formation by thrombin.
Gene References into Functions
  1. In VTE patients the changes of FXIII level and their effect on the risk of VTE show considerable sex-specific differences. Intron K polymorphism results in decreased FXIII levels, but does not influence the risk of VTE. PMID: 28865246
  2. The results suggest that plasma FXIII levels are subjected to multifactorial regulation with age, fibrinogen level and FXIII-B intron K polymorphism being the major determinants. Their effect on FXIII levels might influence the risk of thrombotic diseases. PMID: 27821352
  3. Genetic markers associated with low FXIIIB levels increase risk of ischemic stroke cardioembolic subtype. PMID: 26159793
  4. The FXIII-B intron K nt29756 G allele was associated with significant protection against CAS and MI in patients with a fibrinogen level in the upper tertile. PMID: 25569091
  5. Changes in plasma levels of FXIIIB are associated with cognitive decline in the elderly. PMID: 26088309
  6. Here, we update the knowledge about the pathophysiology of factor XIII deficiency and its therapeutic options. [review] PMID: 24503678
  7. Case Report: congenital FXIII-B deficiency in which alloantibodies developed to exogenous FXIII-B. PMID: 23407795
  8. FXIIIb subunit is found to be within normal range in eight Tunisian famillies with congenital factor XIII deficiency caused by two mutations, while expression of the FXIIIA subunit gene is decreased or undetectable. PMID: 19937244
  9. Develop ELISA/chemoluminescence assay demonstrating that FXIII-A and FXIII-B are low concentration components of tear proteome. PMID: 20079358
  10. role of FXIIIB in modifying catalytic activity of FXIIIA2 during factor XIII mediated crosslinking of fibrinogen PMID: 11816711
  11. F13 B subunit antigen may have a role in susceptibility to stroke based on this study of family members of patients in South Asia PMID: 15634282
  12. Genetic variants of factor XIIIb were evaluated on the effects of survival in myocardial infarction. PMID: 17515963
  13. at least 3 out of the 10 Sushi domains of FXIII-B have the distinct function of forming a homodimer and a heterotetramer, which should be ascribed to the differences in their amino acid sequences PMID: 18652485
  14. A specific colorimetric assay for measuring FXIIIB activity is reported. PMID: 19646949

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Involvement in disease
Factor XIII subunit B deficiency (FA13BD)
Subcellular Location
Secreted.
Database Links

HGNC: 3534

OMIM: 134580

KEGG: hsa:2165

STRING: 9606.ENSP00000356382

UniGene: Hs.435782

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