Human Ficolin-2(FCN2) ELISA kit

Code CSB-EL008551HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
ficolin (collagen/fibrinogen domain containing lectin) 2 (hucolin)
Alternative Names
37 kDa elastin-binding protein ELISA Kit; Collagen/fibrinogen domain containing protein 2 ELISA Kit; Collagen/fibrinogen domain-containing protein 2 ELISA Kit; EBP 37 ELISA Kit; EBP-37 ELISA Kit; EBP37 ELISA Kit; FCN 2 ELISA Kit; Fcn2 ELISA Kit; FCN2_HUMAN ELISA Kit; FCNL ELISA Kit; Ficolin (collagen/fibrinogen domain containing lectin) 2 (hucolin) ELISA Kit; Ficolin B ELISA Kit; Ficolin beta ELISA Kit; Ficolin-2 ELISA Kit; Ficolin-B ELISA Kit; Ficolin-beta ELISA Kit; Ficolin2 ELISA Kit; Hucolin ELISA Kit; L ficolin ELISA Kit; L-ficolin ELISA Kit; OTTHUMP00000022518 ELISA Kit; P35 ELISA Kit; RP11 263F14.2 ELISA Kit; Serum lectin p35 ELISA Kit
Abbreviation
FCN2
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.25 ng/mL-16 ng/mL
Sensitivity
0.06 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human FCN2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1000Average %86
Range %80-94
1:2000Average %95
Range %91-99
1:4000Average %108
Range %98-115
1:8000Average %95
Range %89-98
Recovery
The recovery of human FCN2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9589-98
EDTA plasma (n=4)10198-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
162.007 1.900 1.954 1.844
81.526 1.443 1.485 1.375
41.097 1.009 1.053 0.943
20.645 0.621 0.633 0.523
10.429 0.420 0.425 0.315
0.50.264 0.254 0.259 0.149
0.250.190 0.186 0.188 0.078
00.110 0.109 0.110  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human FCN2 ELISA Kit was designed for the quantitative measurement of Human FCN2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.25 ng/mL-16 ng/mL and the sensitivity is 0.06 ng/mL.

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Target Background

Function
(From Uniprot)
May function in innate immunity through activation of the lectin complement pathway. Calcium-dependent and GlcNAc-binding lectin. Enhances phagocytosis of S.typhimurium by neutrophils, suggesting an opsonic effect via the collagen region.
Gene References into Functions
  1. this study shows that ficolin-2 gene rs7851696 polymorphism is associated with delayed graft function and acute rejection in klidney allograft recipients PMID: 28536887
  2. the minority (C) allele at -64 of the FCN2 gene was less frequent among juvenile idiopathic arthritis patients than among control subjects PMID: 28405017
  3. This is the first study evaluating the FCN2 gene polymorphisms in patients with rheumatic fever and rheumatic carditis finding a protective effect of -986 GG and -4 GG genotypes in the development of rheumatic fever and the -4 AG genotype for the development of carditis. PMID: 28576308
  4. this study did not find any association of FCN2 (encoding ficolin-2 protein) promoter polymorphisms at positions -986, -602, and -4 with dental caries in Polish children. PMID: 28088794
  5. Serum ficolin-2 concentrations in multiple tumor patients are significantly lower than those in healthy donors. PMID: 28844702
  6. this study shows that Ficolin-2 plasma level is associated with short- and long-term mortality in patients with necrotizing soft tissue infection in Denmark PMID: 27355483
  7. The results suggest that the Arctic populations of East Siberia are characterised by specificity of genetic make-up responsible for the activity of L-ficolin. PMID: 28391359
  8. study provides evidence for an important role for the lectin pathway in the inflammatory response induced by cholesterol crystals (CC) and emphasize the role of ficolin-2 and MBL in the CC-mediated inflammation occurring during atherosclerotic plaque development PMID: 27183610
  9. FCN2 inhibits epithelial-mesenchymal transition-induced metastasis of hepatocellular carcinoma via TGF-beta1/Smad signaling. PMID: 27177473
  10. this study shows that FCN2 polymorphisms is not a major risk factor for community-acquired pneumonia in general, but that the +6424G>T SNP in the FCN2 gene predisposes to Coxiella burnetii pneumonia PMID: 28032346
  11. subjects that were heterozygote carriers of both FCN2 + 6424 and FCN3 + 1637delC were sufficient mannan-binding lectin producers PMID: 26795763
  12. systemic lupus erythematosus patients with low plasma ficolin-2 levels had an increased risk of having lupus nephritis PMID: 27981461
  13. Ficolin-2 protein could bind with HIV-1 envelope glycoprotein gp120, and subsequently induce complement dependent cytotoxicity. PMID: 27576476
  14. The FCN2 c.772G>T genotype appears to be associated with predisposition to chronic adenotonsillitis in the pediatric age group. PMID: 27368434
  15. genotype not associated with acute cellular rejection after kidney transplantation, except for a trend toward a deleterious effect of rs7851696 PMID: 26924055
  16. Serum levels of ficolin-2 and ficolin-3 were significantly lower in the cardiac syndrome X patients compared to controls. PMID: 27312152
  17. association of FCN2 polymorphisms with nephritis and severe cumulative damage in SLE patients; no association with rheumatoid arthritis development PMID: 26464189
  18. -557 A>G, -64 A>C and +6424 G>T SNPs of the FCN2 gene were correlated with pulmonary TB. PMID: 26379154
  19. FCN2 and MBL2 allele frequencies were similarly distributed in early and late age-related macular degeneration cases compared with controls PMID: 26207622
  20. this study provide novel insight in the binding and complement activating capacity of the lectin pathway initiation molecules ficolin-2 and ficolin-3 towards relevant Gram-negative pathogens of pathophysiological relevance. PMID: 26074063
  21. Findings indicate the FCN2 variant +6359C>T is associated with the occurrence of visceral leishmaniasis and that ficolin-2 serum levels are elevated in Leishmania infections. PMID: 25965808
  22. L-ficolin modulates the immune response to A. fumigatus. PMID: 25612732
  23. Very low serum ficolin-2 levels were associated with higher risk of 30-day mortality in community-acquired pneumonia patients. PMID: 24736883
  24. hepatitis c virus entry inhibitor PMID: 24854201
  25. paper identifies phosphocholine moieties of pneumococcal teichoic acid as a novel L-ficolin ligand PMID: 25344472
  26. There is lack of association of serum mannose-binding lectin or ficolins with complement activation in patients with antiphospholipid antibodies. PMID: 25083730
  27. MBL deficiency or MBL2 and FCN2 mutations were not associated with an improved hepatitis B vaccine response in Kenyan HIV-1 uninfected individuals. PMID: 25024112
  28. Ficolin-2 was depleted from plasma during cardiac surgery when using heparin-coated bypass circuits and did not reach baseline level 24 h postoperation. PMID: 25174443
  29. CVID patients with bronchiectasis have very low levels of ficolin-2. The reason for the deficiency of ficolin-2 in CVID and any possible causal relationship is currently unknown. PMID: 25251245
  30. binding of ficolin-2 to sulfated/phosphated carbohydrates PMID: 25447524
  31. Ficolin-2 mediates serum protection by recognizing specific O-acetylated epitopes of pneumococcal capsule polysaccharides. PMID: 24683196
  32. MBL2 and FCN2 genotypic variants were analyzed for association with the incidence of acute rejection within the first year after kidney transplantation. PMID: 24486561
  33. Data suggest that the hepatitis C virus (HCV) entry inhibitor ficolin-2 is an antiviral innate immune molecule, whereas apolipoprotein E3 (ApoE3) blocks the effect of ficolin-2 and mediates an immune escape mechanism during chronic HCV infection. PMID: 24928988
  34. bloodstream infections collected prospectively were associated with MBL2 and FCN2 genotypic variants over the first year after kidney transplantation PMID: 24182802
  35. Ficolin-2 defends against virulent Mycobacteria tuberculosis infection in vivo, and its insufficiency is associated with infection in humans. PMID: 24040095
  36. FCN2 polymorphisms for promoter regions -986, -602, -557, -64, -4 and exon 8 regions +6,359, +6,424 were determined in children with B-ALL. Medium/high-risk haplotype were associated with prolonged duration of febrile neutropenia and bacterial infections. PMID: 24453114
  37. results show that SNPS in FCN2 are significantly more susceptible to infectious complications, SIRS and septic shock. PMID: 24227370
  38. Data indicate that Ficolin-2 blood concentration dependents on sampling procedures. PMID: 23911396
  39. Patients with chronic Chagas disease presented with decreased L-ficolin plasma levels that were associated with the 258S polymorphism. PMID: 23593180
  40. this study reports for thefirst time the relationship between full FCN2 genotypes and serumprotein concentrations and discuss the relevance of these findings fordisease association studies PMID: 23619474
  41. Data indicate that in contrast to serum level, the expression of Ficolin-2 (FCN2) was significantly lower in ovarian cancer (OC). PMID: 23744477
  42. Variant FCN2 gene alleles of -64 and +6424 (in strong linkage disequlibrium) are known to be associated with low L-ficolin level or activity. PMID: 23525825
  43. the expected positive association of complement genes with leprosy susceptibility and clinical outcomes in Han Chinese. PMID: 23423485
  44. The association between L-ficolin and thrombocytopenia suggests a pathogenic role for L-ficolin in thrombocytopenia in systemic lupus erythematosus. PMID: 22350641
  45. Our findings suggest that early increased ficolin-2 is highly correlated with hepatic inflammation and rapid viral response. PMID: 23298162
  46. The genotype distribution of three functional SNP variants (-986 G > A, -602 G > A and -4A > G) of ficolin 2 differed significantly between the white, black, and Asian groups. The SNP variants were highly linked to each other. PMID: 22594803
  47. Ficolin-2 Ala258Ser polymorphism in the donor independently predicts improved graft outcome. PMID: 22892990
  48. These findings demonstrate that FCN2 promoter variants (-986G>A and -4A>G) influence ficolin-2 serum levels and susceptibility to schistosomiasis. PMID: 22693230
  49. The purpose of this study was to determine whether circulating levels of ficolin-2 and ficolin-3 are altered in normal pregnancy and pre-eclampsia. PMID: 22670778
  50. Cord blood MBL concentrations were significantly lower in intrauterine-growth-restriction (IUGR) cases than controls. No differences in H- and L-ficolin concentrations were observed between groups. PMID: 22082351

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Subcellular Location
Secreted.
Protein Families
Ficolin lectin family
Tissue Specificity
Expressed by the liver and secreted in plasma.
Database Links

HGNC: 3624

OMIM: 601624

KEGG: hsa:2220

STRING: 9606.ENSP00000291744

UniGene: Hs.54517

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