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Product Name:

Human H-Cadherin,H-Cad/CDH13 ELISA Kit

Product Type : ELISA Kit
Code : CSB-E13817h
Size : 96T,5×96T,10×96T
Uniprot NO. : P55290
Abbreviation : CDH13
Protein Biological Process 1 : Cell Adhesion
Alias : CDHH, H-cadherin|P105|T-cad|T-cadherin|cadherin 13|heart-cadherin|truncated-cadherin
Species : Human
Protein Biological Process 3 : Cell adhesion
Sample Types : serum, plasma and tissue homogenates
Detect Range : 1.25 ng/ml-80 ng/ml
Sensitivity : 0.31 ng/ml
Assay Time : 1-5h
Sample Volume : 50-100ul
Detection Wavelengt : 450 nm
Availability : 3-5 working days
Protocol :
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Target details : This gene is a member of the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region but, unlike the typical cadherin superfamily member, lacks the highly conserved cytoplasmic region. This particular cadherin is a putative mediator of cell-cell interaction in the heart and may act as a negative regulator of neural cell growth. The gene locus is hypermethylated or deleted in breast, ovarian and lung cancers. Two major mRNA transcripts encoding identical proteins are found, products of alternative polyadenylation sites.
HGNC : 1753
RGD : 619745
MGI : 99551
Precision :
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity :
To assess the linearity of the assay, samples were spiked with high concentrations of human H-Cad/CDH13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %93
Range %86-97
1:2Average %92
Range %89-96
1:4Average %102
Range %99-105
1:8Average %94
Range %87-99
Recovery :
The recovery of human H-Cad/CDH13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9793-102
EDTA plasma (n=4)10297-106
Typical Data :
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
802.367 2.235 2.301 2.169
401.756 1.816 1.786 1.654
201.286 1.224 1.255 1.123
100.815 0.801 0.808 0.676
50.464 0.453 0.459 0.327
2.50.323 0.312 0.318 0.186
1.250.228 0.235 0.232 0.100
00.133 0.131 0.132
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