Human Hydroxyacid oxidase 1(HAO1) ELISA kit

Code CSB-EL010127HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
hydroxyacid oxidase (glycolate oxidase) 1
Alternative Names
(S) 2 hydroxy acid oxidase ELISA Kit; Glycolate oxidase ELISA Kit; GOX ELISA Kit; GOX1 ELISA Kit; HAO1 ELISA Kit; HAOX1 ELISA Kit; HAOX1_HUMAN ELISA Kit; Hydroxyacid oxidase 1 ELISA Kit; MGC142225 ELISA Kit; MGC142227 ELISA Kit; OTTHUMP00000030231 ELISA Kit
Abbreviation
HAO1
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
18.75 pg/mL-1200 pg/mL
Sensitivity
4.69 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human HAO1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:5 Average % 98    
Range % 93-112    
1:10 Average % 107    
Range % 103-109    
1:20 Average % 93    
Range % 90-97    
1:40 Average % 88    
Range % 86-94    
Recovery
The recovery of human HAO1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 103 99-106    
EDTA plasma (n=4) 92 90-95    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average Corrected    
1200 2.193 2.369 2.281 2.097    
600 1.700 1.710 1.705 1.521    
300 1.253 1.262 1.258 1.074    
150 0.878 0.898 0.888 0.704    
75 0.532 0.566 0.549 0.365    
37.5 0.411 0.422 0.417 0.233    
18.75 0.231 0.222 0.227 0.043    
0 0.177 0.190 0.184      
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human HAO1 ELISA Kit was designed for the quantitative measurement of Human HAO1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 18.75 pg/mL-1200 pg/mL and the sensitivity is 4.69 pg/mL.

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Target Background

Function
(From Uniprot)
Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2-hydroxy octanoate.
Gene References into Functions
  1. The study presents the first spectroscopic evidence of the formation of an intermediate with absorbance features resembling those of a flavosemiquinone in the oxidative half-reaction of glycolate oxidase. PMID: 21141873
  2. The inhibitor heteroatoms interact with five active-site residues that have been implicated in catalysis in homologous flavodehydrogenases of L-2-hydroxy acids. In addition, the chlorophenyl substituent is surrounded by nonconserved hydrophobic residues. PMID: 20054120
  3. These inhibitions suggest that glycolate binds to the active site of the reduced enzyme, and that DCIP also has affinity for the oxidized enzyme. PMID: 17669354
  4. Active site and loop 4 movements within human glycolate oxidase. PMID: 18215067
Subcellular Location
Peroxisome.
Protein Families
FMN-dependent alpha-hydroxy acid dehydrogenase family
Tissue Specificity
Liver.
Database Links

HGNC: 4809

OMIM: 605023

KEGG: hsa:54363

STRING: 9606.ENSP00000368066

UniGene: Hs.193640

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