Code | CSB-EL013298HU |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
To assess the linearity of the assay, samples were spiked with high concentrations of human MACC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:1 | Average % | 96 | |||||
Range % | 92-101 | ||||||
1:2 | Average % | 84 | |||||
Range % | 80-88 | ||||||
1:4 | Average % | 91 | |||||
Range % | 85-95 | ||||||
1:8 | Average % | 98 | |||||
Range % | 94-102 |
The recovery of human MACC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 106 | 101-113 | |||||
EDTA plasma (n=4) | 86 | 82-90 | |||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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This human MACC1 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human MACC1 in multiple samples, including serum, plasma, tissue homogenates, or cell lysates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the MACC1 levels in the sample and is measured at 450 nm through a microplate reader.
MACC1 has been reported to be overexpressed in multiple cancers including glioblastoma multiforme and gastric cancer and promotes proliferation, metastasis, cancer stem cell-like properties, and drug resistance of cancer cells. It is a potent prognostic biomarker inducing proliferation, migration, invasiveness, and metastasis of cancer cells. MACC1 promotes carcinogenesis, growth, and metastasis of colorectal cancer. Furthermore, MACC1 is linked to the promotion of tumor growth, invasion, and metastasis in gastric cancer, as well as poor prognosis in solid cancers.
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