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Product Name:

Human P53 antibody ELISA Kit

Product Type : ELISA Kit
Code : CSB-E13816h
Size : 96T,5×96T,10×96T
Species : Human
Sample Types : serum, plasma
Detect Range : 1.56 ng/ml-100 ng/ml
Sensitivity : 0.39 ng/ml
Assay Time : 1-5h
Sample Volume : 50-100ul
Detection Wavelengt : 450 nm
Availability : 3-5 working days
Protocol :
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Precision :
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity :
To assess the linearity of the assay, samples were spiked with high concentrations of human P53 antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %92
Range %85-105
1:2Average %94
Range %84-99
1:4Average %99
Range %90-101
1:8Average %92
Range %82-105
Recovery :
The recovery of human P53 antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8983-96
EDTA plasma (n=4)9487-103
Typical Data :
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
1002.689 2.662 2.676 2.570
502.131 2.139 2.135 2.029
251.467 1.479 1.473 1.367
12.50.842 0.884 0.863 0.757
6.250.479 0.487 0.483 0.377
3.120.316 0.304 0.310 0.204
1.560.247 0.254 0.251 0.145
00.107 0.105 0.106
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