Human Protein Wnt-5b(WNT5B) ELISA kit

Product Details

Citations

Related Products

Customer Reviews and Q&A

Target Background

Product Details

Target Name

wingless-type MMTV integration site family, member 5B

Alternative Names

MGC2648 ELISA Kit; Protein Wnt-5b ELISA Kit; Wingless type MMTV integration site family, member 5B ELISA Kit; WNT 5B ELISA Kit; WNT 5B protein ELISA Kit; Wnt family member 5B ELISA Kit; Wnt5b ELISA Kit; WNT5B protein ELISA Kit; WNT5B_HUMAN ELISA Kit

Abbreviation

WNT5B

Uniprot No.

Q9H1J7

Species

Homo sapiens (Human)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

0.156 ng/mL-10 ng/mL

Sensitivity

0.039 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Stem Cells

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of Human WNT5B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-96
1:2	Average %	98
	Range %	94-102
1:4	Average %	94
	Range %	89-98
1:8	Average %	85
	Range %	80-89

Recovery

The recovery of Human WNT5B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-102
EDTA plasma (n=4)	85	80-90

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.663	2.767	2.715	2.616
5	2.326	2.225	2.276	2.177
2.5	1.675	1.578	1.627	1.528
1.25	1.085	1.022	1.054	0.955
0.625	0.507	0.523	0.515	0.416
0.312	0.264	0.276	0.270	0.171
0.156	0.178	0.187	0.183	0.084
0	0.099	0.098	0.099

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human WNT5B ELISA Kit was designed for the quantitative measurement of Human WNT5B protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

Target Background

Function
(From Uniprot)

Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.

Gene References into Functions

exaggerated WNT-5B expression upon cigarette smoke exposure in the bronchial epithelium of COPD patients leads to TGF-beta/Smad3-dependent expression of genes related to airway remodelling PMID: 27126693
the data suggest that WNT5B induces tube formation by regulating the expression of Snail and Slug proteins through activation of canonical and non-canonical WNT signalling pathways. PMID: 27593938
Noncanonical Wnt signaling via Wnt5a/5b/11 may have role in the pathogenesis of aortic valve calcification. PMID: 27932350
WNT-5B induces IL-6 and CXCL8 secretion in pulmonary fibroblasts. PMID: 27036869
overexpression of WNT5B significantly increased cell proliferation, migration and invasion capacities of the COLO 205 cells in vitro. WNT5B may play an important role in the tumorigenesis of colorectal cancer. PMID: 27121420
results suggest that Wnt5b-associated exosomes promote cancer cell migration and proliferation in a paracrine manner PMID: 27762090
these results suggest that TGF-beta1 stimulates HSC-4 cell invasion through the Slug/Wnt-5b/MMP-10 signalling axis. PMID: 26861993
This study identified WNT5B and CTNNBL1 for peak bone mineral density and body composition in males from the Han Chinese ethnic group. PMID: 26733379
The interaction between Wnt isoforms and their LRP6 cognate receptor depends on the jutting loop present in Wnt3a but absent in Wnt5b. PMID: 25425204
Results demonstrate that the Wnt5B pathway may play an important role in atypical teratoid rhabdoid tumor biology. PMID: 25246426
Report increased Wnt5b levels in adriamycin treated liver cancer stem cells within 4h of treatment. PMID: 24944491
This study shows that the WNT5B germline variant rs2010851 was significantly identified as a stage-dependent prognostic marker for CC patients after 5-fluorouracil-based adjuvant therapy. PMID: 23817222
WNT5B is elevated in both in the tumor and the patients' serum from triple negative breast cancer. PMID: 24564888
Knocking down Wnt5b expression reduced phospho-PKCA levels and cell migration. PMID: 24841200
Ursolic acid suppressed the expression of Wnt5alpha/beta and beta-catenin, inducing apoptosis in prostate cancer cells. PMID: 24399733
These results indicate that Wnt5b is involved in the migration ability of OSCC cells through active Cdc42 and RhoA. PMID: 24220306
WNT3 and WNT5B are critical factors, secreted from mesenchymal cancer cells, for instigating the epithelial cancer cell invasion. PMID: 24344732
TGF-beta/Smad, integrin/integrin-linked kinase (ILK) and wnt/beta-catenin signaling pathways are involved in epithelial to mesenchymal transition in human renal fibrogenesis. PMID: 24040439
Differentiation paralleled the activation of Wnt5/Calmodulin signalling by autocrine/paracrine intense secretion of Wnt5a and Wnt5b PMID: 21980498
This is significantly related to fat cell differentiation, suggesting its possible involvement in the onset of diabetes mellitus type 2 and metabolic syndrome. PMID: 21766608
WNT5b is upregulated by beta-estradiol in tumor cell lines PMID: 12165812
WNT5B gene may contribute to conferring susceptibility to type 2 diabetes and may be involved in the pathogenesis of this disease through the regulation of adipocyte function. PMID: 15386214
In leiomyoma cells, the regulation of Wnt5b expression by retinoids appears to be attenuated. PMID: 15972578
Variation in WNT5B predisposes to type 2 diabetes in the absence of obesity. PMID: 18555673

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Subcellular Location

Secreted, extracellular space, extracellular matrix.

Protein Families

Wnt family

Database Links

HGNC: 16265

OMIM: 606361

KEGG: hsa:81029

STRING: 9606.ENSP00000308887

UniGene: Hs.306051

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Code	CSB-EL026139HU
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.