Code | CSB-E13146h |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of human SOST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 88 | ||||
Range % | 80-92 | |||||
1:2 | Average % | 95 | ||||
Range % | 90-100 | |||||
1:4 | Average % | 96 | ||||
Range % | 90-101 | |||||
1:8 | Average % | 93 | ||||
Range % | 88-99 |
The recovery of human SOST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 92 | 89-99 | ||||
EDTA plasma (n=4) | 90 | 85-96 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The human Sclerostin (SOST) ELISA Kit is engineered for accurate measurement of human SOST levels from samples including serum, plasma, or tissue homogenates. It uses the Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the SOST content in the sample. The color intensity is positively correlated with SOST content in the sample. The SOST concentration can be calculated according to the standard curve. This kit is tested with high sensitivity, strong specificity, good linearity, high precision and recovery, as well as lot-to-lot consistency.
SOST is expressed by osteocytes and articular chondrocytes. SOST binds to its receptors on the cell surface of osteoblasts, which suppresses Wnt/β-catenin signaling, thereby inhibiting osteoblast differentiation, proliferation, and activity, leading to reduced osteoblastic bone formation. SOST is essential for normal B lymphocyte development. The absence of SOST causes increased B cell apoptosis and reduced CXCL12, a critical B cell growth-stimulating factor. You et al. showed that SOST is necessary for inducing Th17 cell differentiation, which contributes to bone resorption, through promoting the levels of IL-6 and TFG-β that are related to Th17 differentiation.
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