Product Details

Target Name

sialic acid binding Ig-like lectin 8

Alternative Names

CD329 antigen ELISA Kit; CDw329 ELISA Kit; SAF 2 ELISA Kit; SAF-2 ELISA Kit; SAF2 ELISA Kit; Sialic acid binding Ig like lectin 8 ELISA Kit; Sialic acid-binding Ig-like lectin 8 ELISA Kit; Sialoadhesin family member 2 ELISA Kit; SIGL8_HUMAN ELISA Kit; Siglec 8 ELISA Kit; Siglec-8 ELISA Kit; SIGLEC8 ELISA Kit; SIGLEC8L ELISA Kit

Abbreviation

SIGLEC8

Uniprot No.

Q9NYZ4

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates, cell lysates

Detection Range

0.156 ng/mL-10 ng/mL

Sensitivity

0.039 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human SIGLEC8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	100
	Range %	95-107
1:2	Average %	96
	Range %	92-100
1:4	Average %	84
	Range %	80-92
1:8	Average %	88
	Range %	84-92

Recovery

The recovery of human SIGLEC8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-100
EDTA plasma (n=4)	90	85-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.164	2.246	2.205	2.040
5	1.618	1.634	1.626	1.461
2.5	1.201	1.175	1.188	1.023
1.25	0.792	0.823	0.808	0.643
0.625	0.558	0.571	0.565	0.400
0.312	0.419	0.442	0.431	0.266
0.156	0.324	0.305	0.315	0.150
0	0.162	0.167	0.165

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human SIGLEC8 ELISA Kit was designed for the quantitative measurement of Human SIGLEC8 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

Target Background

Function
(From Uniprot)

Putative adhesion molecule that mediates sialic-acid dependent binding to red blood cells. Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. Recognizes simultaneously epitopes having a terminal N-acetylneuraminic acid (sialic acid) and an underlying 6-O-sulfated galactose. Preferentially binds to Gal-6-sulfated sialyl-Lewis X glycan epitopes.

Gene References into Functions

In differentiating ,eosinophils, SIGLEC-8 messenger RNA and protein were markedly down-regulated in parallel with OLIG2 by an OLIG2 small interfering RNA or a short hairpin RNA. An E-box in the first intron was found to stimulate SIGLEC-8 gene transcription and to bind OLIG2. PMID: 27154355
miR-215 acts in concert with the host gene IARS2 to affect neuron migration and proliferation through the target gene SIGLEC-8. PMID: 28006787
Our study suggested that intratumoral Siglec-8 expression was an independent prognostic factor for overall survival of patients with gastric cancer PMID: 26883254
Siglec-8 expression is sensitive to tiotropium and formoterol, indicating that it may be involved in COPD pathogenesis and may influence COPD phenotyping PMID: 23835953
In activated eosinophils ligation of Siglec-8 leads to ROS-dependent enhancement of IL-5-induced ERK phosphorylation, which results in a novel mode of biochemically regulated eosinophil cell death. PMID: 23684072
Engagement of Siglec-8 on blood eosinophils results in caspase- and mitochondria-dependent apoptosis. PMID: 22324980
Report the development of a sensitive competitive ELISA to detect sSiglec-8 in human serum samples obtained from patients with various forms of eosinophilia. PMID: 22683541
Eosinophils primed by IL-33 and/or IL-5 in vivo would be expected to display enhanced susceptibility to undergoing Siglec-8-induced apoptosis. PMID: 22079334
expressed on eosinophils and basophils from subjects with chronic eosinophilic leukemia, chronic myelogenous leukemia, and on malignant and non-malignant bone marrow mast cells PMID: 21938510
The Siglec-8 gene may be a susceptibility locus for asthma. PMID: 20087405
Sialic acid binding immunoglobulin-like lectin 8 (Siglec-8) cross-linking with antibodies rapidly generated caspase-3-like activity and reduced eosinophil viability through induction of apoptosis PMID: 12609831
Siglec-8 binds preferentially to a sLex structure bearing an additional sulfate ester on the galactose 6-hydroxyl PMID: 15563466
Siglec-8-induced apoptosis in eosinophils occurs through the sequential production of reactive oxygen species, followed by induction of mitochondrial injury and caspase cleavage. PMID: 16157303
IL-5 priming enhances Siglec-8-mediated mitochondrial and ROS-dependent eosinophil apoptosis and eliminates caspase dependence. PMID: 17690326
First reported inhibitory effects of Siglec engagement on human mast cells. PMID: 18036650
A review of the cytotoxic effects of natural anti-Siglec8 autoantibodies on both neutrophils and eosinophils. PMID: 18558361
REVIEW: Siglec-8 and Siglec-F function on human eosinophils, and Siglec-8 function on mast cells PMID: 19178537
Selective and specific engagement and binding of Siglec-8 on eosinophils by a synthetic 6'-sulfo-sLe(x)-containing ligand could be useful for controlling eosinophilic inflammatory responses in vivo. PMID: 19458105

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Subcellular Location

Membrane; Single-pass type I membrane protein.

Protein Families

Immunoglobulin superfamily, SIGLEC (sialic acid binding Ig-like lectin) family

Tissue Specificity

Expressed specifically on red blood cells namely basophil, mast cells and eosinophils.

Database Links

HGNC: 10877

OMIM: 605639

KEGG: hsa:27181

STRING: 9606.ENSP00000321077

UniGene: Hs.447899

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Human Sialic acid-binding Ig-like lectin 8(SIGLEC8) ELISA kit

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Target Background