Product Details

Target Name

slit homolog 3 (Drosophila)

Alternative Names

FLJ10764 ELISA Kit; KIAA0814 ELISA Kit; MEGF 5 ELISA Kit; MEGF5 ELISA Kit; Multiple EGF-like domains protein 5 ELISA Kit; Multiple epidermal growth factor like domains 5 ELISA Kit; Multiple epidermal growth factor-like domains protein 5 ELISA Kit; SLIL 2 ELISA Kit; SLIL2 ELISA Kit; SLIT 1 ELISA Kit; Slit 2 ELISA Kit; Slit 3 ELISA Kit; Slit homolog 3 (Drosophila) ELISA Kit; Slit homolog 3 ELISA Kit; Slit homolog 3 protein ELISA Kit; Slit-3 ELISA Kit; SLIT1 ELISA Kit; Slit2 ELISA Kit; Slit3 ELISA Kit; SLIT3_HUMAN ELISA Kit

Abbreviation

SLIT3

Uniprot No.

O75094

Species

Homo sapiens (Human)

Sample Types

serum, plasma, cell culture supernates

Detection Range

0.156 ng/mL-10 ng/mL

Sensitivity

0.039 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Neuroscience

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human SLIT3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	90-97
1:2	Average %	85
	Range %	82-93
1:4	Average %	110
	Range %	105-115
1:8	Average %	106
	Range %	102-117

Recovery

The recovery of human SLIT3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	86-95
EDTA plasma (n=4)	99	95-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	1.866	1.955	1.911	1.777
5	1.472	1.588	1.530	1.396
2.5	1.162	1.201	1.182	1.048
1.25	0.864	0.873	0.869	0.735
0.625	0.525	0.558	0.542	0.408
0.312	0.312	0.313	0.313	0.179
0.156	0.267	0.276	0.272	0.138
0	0.137	0.131	0.134

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human SLIT3 ELISA Kit was designed for the quantitative measurement of Human SLIT3 protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

Code	CSB-EL021769HU
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Human Slit homolog 3 protein(SLIT3) ELISA kit

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