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- A 96-well Assay plate --The 96-well plate has been pre-coated with anti-human VB12 antibody.
- Standard (2 x 0.5 ml)--Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
- Biotin-conjugated VB12(100 x concentrate) (1 x 600 μl)
- HRP-avidin (100 x concentrate) (1 x 120 μl)
- Biotin-conjugate Diluent (1 x 10 ml) --Dilute the Biotin-conjugated VB12
- HRP-avidin Diluent (1 x 20 ml) --Dilute the HRP-avidin solution.
- Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration.
- Wash Buffer (25x concentrate) (1 x 20 ml)--Wash away unbound or free substances.
- TMB Substrate (1x 10 ml) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- Stop Solution (1 x 10ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells)
- An Instruction manual
