Human Xin actin-binding repeat-containing protein 1(XIRP1) ELISA kit

Code CSB-EL026195HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
xin actin-binding repeat containing 1
Alternative Names
AI415219 ELISA Kit; Cardiomyopathy-associated protein 1 ELISA Kit; CMYA1 ELISA Kit; DKFZp451D042 ELISA Kit; DKFZp779C1255 ELISA Kit; DKFZp779C1947 ELISA Kit; MGC144145 ELISA Kit; MGC144146 ELISA Kit; Xin actin-binding repeat-containing protein 1 ELISA Kit; XIN ELISA Kit; Xirp1 ELISA Kit; XIRP1_HUMAN ELISA Kit
Abbreviation
XIRP1
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
31.25 pg/mL-2000 pg/mL
Sensitivity
7.8 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human XIRP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %96
Range %90-102
1:2Average %90
Range %84-95
1:4Average %99
Range %95-106
1:8Average %97
Range %90-104
Recovery
The recovery of human XIRP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8893-96
EDTA plasma (n=4)10198-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20001.917 1.947 1.932 1.750
10001.286 1.299 1.293 1.111
5000.804 0.815 0.810 0.628
2500.536 0.505 0.521 0.339
1250.401 0.435 0.418 0.236
62.50.312 0.323 0.318 0.136
31.250.247 0.261 0.254 0.072
00.181 0.183 0.182  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human XIRP1 ELISA Kit was designed for the quantitative measurement of Human XIRP1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL .

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Target Background

Function
(From Uniprot)
Protects actin filaments from depolymerization.
Gene References into Functions
  1. Abnormal CMYA1 expression affects the phosphorylation of Cx43 through the protein kinase c signaling pathway, which is involved in the regulation of gap junction intercellular communication. PMID: 29176328
  2. Aciculin interacts with filamin C and Xin and is essential for myofibril assembly. PMID: 24963132
  3. The strong correlation between the degree of muscle damage and Xin immunoreactivity suggests that Xin may be a suitable outcome measure to evaluate disease progression and treatment effects in clinical trials. PMID: 24225086
  4. We identify the SH3 domains of nebulin and nebulette as novel ligands of proline-rich regions of Xin and XIRP2. PMID: 23985323
  5. Data provide further support that ALS2CL, EPHA3, and CMYA1 are bona-fide tumor-suppressor genes and contribute to the tumorigenesis of HNSCC. PMID: 20657180
  6. Here, we identify Xin, the protein encoded by the human gene 'cardiomyopathy associated 1'(CMYA1) as filamin c binding partner at these specialized structures where the ends of myofibrils are attached to the sarcolemma PMID: 16631741
  7. Data show that in myofibrillar myopathies Xin protein exhibites significant alterations in their localization. PMID: 19151983

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Subcellular Location
Cell junction. Note=Colocalizes with actin stress fibers.
Protein Families
Xin family
Tissue Specificity
Isoform A, isoform B and isoform C are expressed in heart.
Database Links

HGNC: 14301

OMIM: 609777

KEGG: hsa:165904

STRING: 9606.ENSP00000343140

UniGene: Hs.447868

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