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Product Name:

Human anti-Mullerian hormone (AMH) ELISA kit

Product Type : ELISA Kit
Code : CSB-E12756h
Size : 96T,5×96T,10×96T
Uniprot NO. : P03971
Abbreviation : AMH
Alias : MIF, MIS, Mullerian inhibiting factor|Mullerian inhibiting substance
Species : Human
Sample Types : serum, plasma, tissue homogenates
Detect Range : 0.375 ng/ml-150 ng/ml
Sensitivity : 0.375ng/ml
Assay Time : 1-5h
Sample Volume : 50-100ul
Detection Wavelengt : 450 nm
Availability : 3-5 working days
Protocol :
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Target details : Anti-Mullerian hormone is a member of the transforming growth factor-beta gene family which mediates male sexual differentiation. Anti-Mullerian hormone causes the regression of Mullerian ducts which would otherwise differentiate into the uterus and fallopian tubes. Some mutations in the anti-Mullerian hormone result in persistent Mullerian duct syndrome.
HGNC : 464
RGD : 2108
MGI : 88006
BIOWWW : AMH
Precision :
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity :
To assess the linearity of the assay, samples were spiked with high concentrations of human AMH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %90
Range %85-96
1:2Average %96
Range %90-108
1:4Average %98
Range %95-100
1:8Average %97
Range %90-105
Recovery :
The recovery of human AMH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9589-101
EDTA plasma (n=4)9790-105
Typical Data :
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2Average
1500.495 0.484 0.490
28.120.796 0.781 0.789
4.691.292 1.282 1.287
1.311.494 1.487 1.491
0.3751.741 1.745 1.743
02.569 2.523 2.546
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