Product Details

Target Name

fibroblast growth factor 13

Alternative Names

FGF 13 ELISA Kit; FGF 2 ELISA Kit; FGF-13 ELISA Kit; FGF13 ELISA Kit; FGF13_HUMAN ELISA Kit; FGF2 ELISA Kit; FHF 2 ELISA Kit; FHF-2 ELISA Kit; FHF2 ELISA Kit; Fibroblast growth factor 13 ELISA Kit; Fibroblast growth factor homologous factor 2 ELISA Kit; OTTHUMP00000024143 ELISA Kit; OTTHUMP00000024144 ELISA Kit

Abbreviation

FGF13

Uniprot No.

Q92913

Species

Homo sapiens (Human)

Sample Types

serum, plasma, cell culture supernates

Detection Range

12.5 pg/mL-800 pg/mL

Sensitivity

3.12 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human FGF-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-90
1:2	Average %	96
	Range %	92-100
1:4	Average %	90
	Range %	85-94
1:8	Average %	92
	Range %	88-96

Recovery

The recovery of human FGF-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	84-92
EDTA plasma (n=4)	104	100-109

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
800	2.152	2.263	2.208	2.062
400	1.607	1.712	1.660	1.514
200	1.179	1.096	1.138	0.992
100	0.781	0.745	0.763	0.617
50	0.435	0.451	0.443	0.297
25	0.314	0.307	0.311	0.165
12.5	0.228	0.234	0.231	0.085
0	0.143	0.149	0.146

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human FGF13 ELISA Kit was designed for the quantitative measurement of Human FGF13 protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 12.5 pg/mL-800 pg/mL and the sensitivity is 3.12 pg/mL.

Related Products

Target Background

Function
(From Uniprot)

Microtubule-binding protein which directly binds tubulin and is involved in both polymerization and stabilization of microtubules. Through its action on microtubules, may participate in the refinement of axons by negatively regulating axonal and leading processes branching. Plays a crucial role in neuron polarization and migration in the cerebral cortex and the hippocampus. May regulate voltage-gated sodium channels transport and function. May also play a role in MAPK signaling. Required for the development of axonal initial segment-targeting inhibitory GABAergic synapses made by chandelier neurons.

Gene References into Functions

The present study reported the presence of FGF13 in the follicular fluid of women undergoing IVF/ICSI. Moreover, the relationships between FF-FGF13 and FF-TT, ovarian morphology and oocyte developmental competence imply that FF-FGF13 might be involved in the pathophysiological process of polycystic ovary syndrome. PMID: 30257687
Autism-related protein MeCP2 regulates FGF13 expression and emotional behaviors PMID: 27916441
FGF13 was downregulated in human placentae with early-onset preeclampsia. FGF13 played an important role in maintaining placental trophoblast permeability via the modulation of E-cadherin. PMID: 29405966
We propose that, in cells in which activated oncogenes drive excessive protein synthesis, FGF13 may favor survival by maintaining translation rates at a level compatible with the protein quality-control capacity of the cell. Thus, FGF13 may serve as an enabler, allowing cancer cells to evade proteostasis stress triggered by oncogene activation. PMID: 27994142
Study screened the coding and splice site regions of the FGF13 gene in a sample of 45 unrelated probands where genetic epilepsy with febrile seizures plus segregated in an X-linked pattern; subsequently identified a de novo FGF13 missense variant in an additional patient with febrile seizures and facial edema. Results suggest that FGF13 is not a common cause of genetic epilepsy with febrile seizures plus. PMID: 27810516
identified two novel native phosphorylation sites in the C terminus of NaV1.5 that impair FGF13-dependent regulation of channel inactivation and may contribute to CaMKIIdeltac-dependent arrhythmogenic disorders in failing hearts. PMID: 28882890
These data showed the diversity of the roles of the FGF13 N-termini in NaV1.5 channel modulation and suggested the importance of isoform-specific regulation PMID: 27246624
the FGF13/Nav1.7 complex is essential for sustaining the transmission of noxious heat signals PMID: 28162808
for PCa patients after RP, FGF13 serves as a potential novel prognostic marker that improves prediction of BCR-free survival, in particular if combined with other clinical parameters. PMID: 26891277
The findings of this study reveal a novel cause of this syndrome and underscore the powerful role of FGF13 in control of neuronal excitability. PMID: 26063919
X-chromosome deletions may be a cause of WS with larger deletions being lethal to males and that FGF13 mutations may be a cause of Wildervanck Syndrome (WS). PMID: 23373430
Upregulated expression of FGF13/FHF2 mediates resistance to platinum drugs in cervical cancer cells PMID: 24113164
FGF13 has a role in hair follicle growth and in the hair cycle as shown in a family with X-linked congenital generalized hypertrichosis PMID: 23603273
interactions with perlecans PMID: 11847221
identification of a domain controlling angiogenic properties PMID: 12496262
FHF2B is an interacting partner of Na(v)1.6. The preferential expression of FHF2B in sensory neurons may provide a basis for physiological differences in sodium currents that have been reported at the nodes of Ranvier in sensory versus motor axons. PMID: 15282281

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Subcellular Location

Cell projection, filopodium. Cell projection, growth cone. Cell projection, dendrite. Nucleus. Cytoplasm.; [Isoform 1]: Nucleus, nucleolus.; [Isoform 2]: Cytoplasm. Nucleus.

Protein Families

Heparin-binding growth factors family

Tissue Specificity

Ubiquitously expressed. Predominantly expressed in the nervous system.

Database Links

HGNC: 3670

OMIM: 300070

KEGG: hsa:2258

STRING: 9606.ENSP00000359635

UniGene: Hs.6540

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Code	CSB-E13849h
Size	96T,5×96T,10×96T
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Human fibroblast growth factor-13,FGF-13 ELISA Kit

Product Details

Citations

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Target Background