Human platelet activating factor,PAF ELISA Kit

Code CSB-E07929h
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
platelet activating factor,PAF
Alternative Names
N/A
Abbreviation
PAF
Species
Homo sapiens (Human)
Sample Types
serum, urine, cell culture supernates, tissue homogenates, cell lysates
Detection Range
4.69 ng/mL-300 ng/mL
Sensitivity
1.17 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Biochemicals
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human PAF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 85  
Range % 81-92  
1:2 Average % 95  
Range % 90-105  
1:4 Average % 94  
Range % 91-102  
1:8 Average % 90  
Range % 80-98  
Recovery
The recovery of human PAF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 91 88-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
300 2.097 2.093 2.095 1.994  
150 1.823 1.793 1.808 1.707  
75 1.476 1.374 1.425 1.324  
37.5 1.029 0.986 1.008 0.907  
18.75 0.782 0.772 0.777 0.676  
9.38 0.421 0.401 0.411 0.310  
4.69 0.218 0.202 0.210 0.109  
0 0.105 0.097 0.101    
Materials provided
  • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human PAF antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
  • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • One vial Biotin-labeled PAF antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
  • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
  • One vial Biotin-conjugateDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
  • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
  • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
  • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
  • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
  • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

The Human Platelet Activating Factor (PAF) ELISA Kit is a powerful tool for quantitative detection of PAF in various sample types. PAF, also known as a platelet-activating factor, is a bioactive lipid mediator that plays a crucial role in inflammation and immune response.

This kit is designed for the detection of human PAF in serum, urine, cell culture supernates, tissue homogenates, and cell lysates. Based on a sandwich assay principle, our kit provides accurate and reproducible results with minimal interference from other proteins.

With a sensitivity of 1.17 ng/mL and a detection range of 4.69 ng/mL-300 ng/mL, this ELISA kit can accurately measure PAF levels in various sample types. The assay time ranges from 1-5 hours, and the sample volume required is 50-100ul. The detection wavelength is 450 nm, ensuring accurate and precise measurement of PAF levels.

This PAF ELISA Kit has been cited in more than 8 publications, demonstrating its effectiveness and reliability in research studies. It is an essential tool for researchers studying the role of PAF in various diseases, such as asthma, sepsis, and cardiovascular disease.

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