Code | CSB-E05125h |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of human sRANKL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:5 | Average % | 94 | ||||
Range % | 90-99 | |||||
1:10 | Average % | 87 | ||||
Range % | 82-92 | |||||
1:20 | Average % | 92 | ||||
Range % | 88-97 | |||||
1:40 | Average % | 90 | ||||
Range % | 85-94 |
The recovery of human sRANKL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 90 | 86-94 | ||||
EDTA plasma (n=4) | 95 | 90-99 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The product CSB-E05125h is a ready-to-use microwell, strip plate ELISA kit for quantitatively analyzing the presence of TNFSF11 in different biological samples, including serum, plasma, and tissue homogenates. This TNFSF11 ELISA kit specifically recognizes the human TNFSF11 in the sample. It adopts the Sandwich ELISA technique in conjunction with antibody-antigen specific binding and an enzyme colorimetric reaction to indicate the presence of human TNFSF11 and quantitatively measure the concentration of TNFSF11 in the samples.
TNFSF11, also known as RANKL, is an osteoclastogenic cytokine that combines bone with the immune system. RANKL/RANK signaling regulates osteoclast development, bone metabolism, lymph node development, and T cell/dendritic cell communication. In addition to modulating bone resorption and remodeling, the RANKL/RANK axis is also involved in many other cellular processes, including immunity, proliferation and division of mammary epithelial cells, and mammary gland formation during lactation. Studies have shown that RANKL/RANK is involved in each stage of cancer development, from pre-cancerous lesions to the establishment of metastases.
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