Product Details

Target Name

thyrotropin-releasing hormone

Alternative Names

TRH ELISA Kit; Pro-thyrotropin-releasing hormone ELISA Kit; Pro-TRH ELISA Kit; Prothyroliberin) [Cleaved into: Thyrotropin-releasing hormone ELISA Kit; TRH ELISA Kit; Protirelin ELISA Kit; TSH-releasing factor ELISA Kit; Thyroliberin ELISA Kit; Thyrotropin-releasing factor ELISA Kit; TRF)] ELISA Kit

Abbreviation

TRH

Uniprot No.

P20396

Species

Homo sapiens (Human)

Sample Types

serum, plasma

Detection Range

2.5 pg/mL-100 pg/mL

Sensitivity

1.25 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human TRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	81-92
1:2	Average %	95
	Range %	85-105
1:4	Average %	97
	Range %	92-102
1:8	Average %	93
	Range %	86-98

Recovery

The recovery of human TRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	97	91-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
100	2.232	2.252	2.242
50	1.196	1.156	1.176
25	0.635	0.648	0.642
12.5	0.357	0.362	0.360
5	0.146	0.151	0.149
2.5	0.075	0.079	0.077

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human TRH ELISA Kit was designed for the quantitative measurement of Human TRH protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 2.5 pg/mL-100 pg/mL and the sensitivity is 1.25 pg/mL.

Target Background

Function
(From Uniprot)

As a component of the hypothalamic-pituitary-thyroid axis, it controls the secretion of thyroid-stimulating hormone (TSH) and is involved in thyroid hormone synthesis regulation. It also operates as modulator of hair growth. It promotes hair-shaft elongation, prolongs the hair cycle growth phase (anagen) and antagonizes its termination (catagen) by TGFB2. It stimulates proliferation and inhibits apoptosis of hair matrix keratinocytes.

Gene References into Functions

TRH rs13097335 may have a protective role toward the development of breast cancer. PMID: 25362934
Data indicate that decrease of cholesterol level impaired the functional coupling between the thyrotropin-releasing hormone receptor and the cognate G proteins. PMID: 25485475
Cpe mutations in mouse may affect processing of prothyrotropin releasing hormone into thyrotropin releasing hormone. PMID: 12270926
TRH inhibits melanin-concentrating hormone (MCH) neurons by increasing synaptic inhibition from local GABA neurons. PMID: 22378876
This study introduces TRH as a novel, potent, selective, and evolutionarily highly conserved neuroendocrine factor controlling human pigmentation in situ. PMID: 21956127
Case Report: Isolated idiopathic central hypothyroidism in an adult, possibly caused by thyrotropin releasing hormone (TRH) deficiency. PMID: 20687402
Thus, human scalp hair follicles are both a source and a target of TRH, which operates as a potent hair-growth stimulator PMID: 19825978
TRH mRNA levels in "mute" thyrotropinomas: cause-effect or coincidence? PMID: 12067454
results show that that leptin can regulate trh gene expression via activation of intracellular signal transducer and activator of transcription 3 (STAT3) proteins in TRH neurons in the hypothalamus PMID: 14764629
The presence of TRH in dysplastic nevi may be predictive for the development of melanoma. PMID: 15328193
Our results support the hypothesis that patients with pseudohypoparathyroidism Ia have impaired sensitivity to both TSH and TRH. PMID: 18805917

Hide All

Subcellular Location

Secreted.

Protein Families

TRH family

Tissue Specificity

Hypothalamus. Expressed in the hair follicle epithelium (at protein level).

Database Links

HGNC: 12298

OMIM: 613879

KEGG: hsa:7200

STRING: 9606.ENSP00000303452

UniGene: Hs.182231

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-E08146h
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Human thyrotropin-releasing hormone,TRH ELISA KIT

Product Details

Related Products

Customer Reviews and Q&A

Target Background