Mouse Agrin(AGRN) ELISA kit

Product Details

Related Products

Customer Reviews and Q&A

Target Background

Product Details

Target Name

agrin

Alternative Names

Agrn ELISA kit; AgrinAgrin [Cleaved into: Agrin N-terminal 110 kDa subunit; Agrin C-terminal 110 kDa subunit; Agrin C-terminal 90 kDa fragment ELISA kit; C90); Agrin C-terminal 22 kDa fragment ELISA kit; C22)] ELISA kit

Abbreviation

AGRN

Uniprot No.

A2ASQ1

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.312 ng/mL-20 ng/mL

Sensitivity

0.078 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Neuroscience

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse AGRN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	81-92
1:2	Average %	102
	Range %	95-106
1:4	Average %	93
	Range %	85-96
1:8	Average %	97
	Range %	91-100

Recovery

The recovery of mouse AGRN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	82-93
EDTA plasma (n=4)	98	90-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.257	2.166	2.212	2.038
10	1.361	1.378	1.370	1.196
5	0.858	0.884	0.871	0.697
2.5	0.572	0.578	0.575	0.401
1.25	0.447	0.433	0.440	0.266
0.625	0.339	0.354	0.347	0.173
0.312	0.248	0.232	0.240	0.066
0	0.176	0.172	0.174

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Mouse AGRN ELISA Kit was designed for the quantitative measurement of Mouse AGRN protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

Target Background

Function
(From Uniprot)

heparan sulfate basal lamina glycoprotein that plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ) and directs key events in postsynaptic differentiation. This neuron-specific (z+) isoform is a component of the AGRN-LRP4 receptor complex that induces the phosphorylation and activation of MUSK. The activation of MUSK in myotubes induces the formation of NMJ by regulating different processes including the transcription of specific genes and the clustering of AChR in the postsynaptic membrane. Calcium ions are required for maximal AChR clustering. AGRN function in neurons is highly regulated by alternative splicing, glycan binding and proteolytic processing. Modulates calcium ion homeostasis in neurons, specifically by inducing an increase in cytoplasmic calcium ions. Functions differentially in the central nervous system (CNS) by inhibiting the alpha(3)-subtype of Na+/K+-ATPase and evoking depolarization at CNS synapses. This transmembrane agrin (TM-agrin) isoform, the predominate form in neurons of the brain, induces dendritic filopodia and synapse formation in mature hippocampal neurons in large part due to the attached glycosaminoglycan chains and the action of Rho-family GTPases.; Isoform 2 and isoform 3: these isoforms lacking the 'z' insert (z0) are muscle-specific, have no AChR clustering ability and may be involved in nervous system endothelial cell differentiation.; involved in regulation of neurite outgrowth probably due to the presence of the glycosaminoglcan (GAG) side chains of heparan and chondroitin sulfate attached to the Ser/Thr- and Gly/Ser-rich regions. Also involved in modulation of growth factor signaling.; this released fragment is important for agrin signaling and to exert a maximal dendritic filopodia-inducing effect. All 'z' splice variants (z+) of this fragment also show an increase in the number of filopodia.

Gene References into Functions

The presence of agrin at locations with particular importance for the growth and stability of atherosclerotic plaques renders this molecule strategically positioned to influence plaque development and vulnerability PMID: 29405249
Agrin induces clustering and activation of EphB1 receptors on developing erythroblasts, leading to the activation of alpha5beta1 integrins. PMID: 26990660
identificaton of agrin, a component of neonatal extracellular matrix, as required for the full regenerative capacity of neonatal mouse hearts PMID: 28581497
The presence of C-terminal fragment of agrin (CAF) in urine suggests either glomerular filtration or secretion into urine. PMID: 27380275
Agrin strongly promotes chondrocyte differentiation and cartilage formation in vivo. PMID: 26290588
Reveal novel roles for APP in regulating neuromuscular synapse formation through hetero-oligomeric interaction with LRP4 and agrin and thereby provide new insights into the molecular mechanisms that govern NMJ formation and maintenance. PMID: 23986861
Agrin is frequently upregulated and important for oncogenic property of hepatocellular carcinoma. PMID: 25630468
agrin contributes to barrier characteristics of brain endothelium. PMID: 25107608
NMJs in agrin-deficient mice rapidly disappear after birth. Agrin is essential for postnatal NMJ maintenance, but not for their embryonic formation, and also for the postnatal, but not prenatal, midmuscle localization of postsynaptic specializations. PMID: 25368159
reviews the evidence that agrin regulates synapse development, plasticity and signaling in the brain and discusses the evidence for the proposed mechanisms PMID: 22414531
Using gamma-secretase drug inhibitors and presenilin mutants in embryonic fibroblasts, we found that a mature gamma-secretase complex was required to repress neurotrypsin expression and agrin cleavage. PMID: 24145027
Neural agrin slows the degradation of surface AChR in cultured muscle cellsb via phosphorylation of the AChR beta subunit at Y390, stabilizing AChR at developing synapses & adult neuromuscular junctions. PMID: 23325468
Agrin is Required for Maintenance of the Postsynaptic Apparatus. Agrin Loss Disrupts Nerve Terminal Organization PMID: 23056392
Two classic synaptic signalling systems (neural agrin and neuregulin-1) converge upon MuSK to regulate postsynaptic differentiation. PMID: 22328506
Agrin induces association of Chrna1 mRNA and nicotinic acetylcholine receptor in C2C12 myotubes. PMID: 22884571
The suppression of agrin reduced hyperambulation and holeboard exploration, restored anxiety-like behavior (or reduced risk-taking behavior), improved prepulse inhibition, shortened the circadian period and increased brain Na(+) ,K(+) -ATPase activity. PMID: 22520507
the cascade linking agrin to CLASP2-mediated microtubule capturing at the synaptic membrane is essential for the maintenance of a normal neuromuscular phenotype PMID: 22851317
Agrin deficiency impairs monocytic cell maturation. PMID: 22517892
Studies of neurotrypsin-deficient and agrin-overexpressing mice revealed that old-age sarcopenia also develops without neurotrypsin and is not prevented by elevated levels of agrin. Define neurotrypsin- and age-dependent sarcopenia as 2 distinct entities. PMID: 21885656
The Agrn point mutation is a model for congenital myasthenic syndromes caused by Agrn mutations and potentially other related neuromuscular diseases. PMID: 21890498
These results indicate that AGRIN is important for maintaining blood-brain barrier composition and that changes in Agrn expression (particularly vessel-associated AGRIN) influence Abeta homeostasis in mouse models of Alzheimer disease. PMID: 22082664
Many clusters of acetylcholine receptors are observed in nestin/agrin double knockout-out mutants compared to significantly fewer in agrin single knockouts. PMID: 21832185
The results support influence of agrin on AQP4, orthogonal arrays of particles and volume regulation. PMID: 20920487
The agrin C22 construct, constitutes a well-folded, stable domain, while the deletion of 48 residues that correspond to strands beta1-beta4 of the agrin laminin G3 domain imposed by the agrin C15 construct leads to a misfolded protein. PMID: 21037280
A role is proposed for agrin and laminin binding to alpha-dystrogycan at sensory synapses that places this activity upstream of neuregulin-ErbB signaling. PMID: 20213761
the N-terminal half of agrin is involved in agrin's ability to inhibit neurite outgrowth. PMID: 11793362
regulates neuronal responses to excitatory neurotransmitters PMID: 11817901
Agrin plays an organizing role in the formation of sympathetic synapses PMID: 12221070
Structures required for agrin signaling in CNS neurons are contained within a 20-kD COOH-terminal fragment of the protein. Distinct regions within agrin are responsible for receptor binding and activation. PMID: 12796478
Expression of agrin in non-synaptic region varies greatly between different muscles in wild-type mice and its levels are altered in dystrophic muscle. PMID: 12798796
Agrn becomes concentrated at neuroeffector and neuromuscular junctions in developing urinary bladder. PMID: 15711988
agrin and perlecan cluster with laminin-2 to contribute to assembly of aneural AChR clusters that precede neural agrin release as well as affect later NMJ development PMID: 16219760
agrin is able to rapidly and fully negate the laminin substrate clustering effect while inducing the rapid formation of new clusters. Acetylcholine receptor (AChR) half-life is not affected by agrin despite its role in inducing aggregation of AChRs. PMID: 16256100
These data provide the first evidence for a specific role of TM-agrin in shaping the cytoskeleton of neurites in the developing nervous system. PMID: 16364653
findings strongly indicate that agrin and alpha-dystroglycan mediate lymphocyte activation; furthermore, agrin-involved lymphocyte activation is mediated by alpha-dystroglycan PMID: 16394267
These data demonstrate that, through its interaction with the alpha3 sodium-potassium ATPase, agrin regulates activity-dependent processes in neurons, providing a molecular framework for agrin action in the CNS. PMID: 16630822
agrin likely triggers AChR clustering or maintains clusters through the coalescence of lipid rafts which play a pivotal role in the assembly of the postsynaptic membrane at the neuromuscular junction upon agrin signaling PMID: 16816402
Data show that agrin is highly expressed by chondrocytes and localizes to the growth plate in wild-type mice and plays a critical role in normal skeletal growth. PMID: 17186266
Transgenic mice overexpressing the heparan sulfate proteoglycan agrin have severe ocular dysgenesis. PMID: 17196957
agrin is required for proper structural and functional development of an interneuronal synapse in vivo PMID: 17443806
dysregulation of agrin processing is a good candidate for a pathogenetic mechanism underlying mental retardation PMID: 17586728
Although agrin contributes significantly to the anionic charge to the GBM, both it and its charge are not needed for glomerular permselectivity. PMID: 17591961
Agrin plays an important role in the formation and/or the maintenance of excitatory synapses in the brain. PMID: 17611272
The acetylcholine receptor-associated protein rapsyn interacted with calpain in an agrin-dependent manner, and this interaction inhibited the protease activity of calpain. PMID: 17640526
neurotrypsin exclusively cleaves glycanated variants of agrin; neurotrypsin-dependent cleavage of agrin was concentrated at synapses, where the most heavily glycanated variants of agrin predominate PMID: 18230682
Our study reveals a potentially novel mechanism that regulates agrin/MuSK signaling cascade. PMID: 18272689
At the postnatal neuromuscular synapse agrin signaling elevates the expression and targeting of rapsyn to the postsynaptic membrane, thereby packing more AChRs into stable, functionally-important AChR aggregates. PMID: 18506821
Muscle-wide secretion of a miniaturized form of neural agrin rescues focal neuromuscular innervation in agrin mutant mice. PMID: 18685098
These data are consistent with a role for Pofut1 in AChR aggregation during synaptogenesis via the regulation of the synaptogenic activity of muscle agrin. PMID: 18775496
These experiments indicate that Lrp4 is the long-sought and elusive receptor for Agrin and has a critical role in activating MuSK and stimulating neuromuscular synapse formation. PMID: 18848351

Hide All

Subcellular Location

Cell junction, synapse. Cell membrane; Single-pass type II membrane protein.

Tissue Specificity

Expressed in central nervous system (CNS) synapses such as in the cerebral cortex and hippocampus. Localizes to basal lamina of hippocampal blood vessels. Both (z+) and (z-) isoforms found in kidney, heart and cerebral vasculature.

Database Links

STRING: 10090.ENSMUSP00000137931

UniGene: Mm.273098

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-EL001461MO
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.