Mouse Ectodysplasin-A(EDA) ELISA kit

Code CSB-EL007389MO
Size 96T,5×96T,10×96T
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Product Details

Target Name
ectodysplasin A
Alternative Names
Eda; Ed1; Ta; Ectodysplasin-A; EDA protein homolog; Tabby protein
Abbreviation
EDA
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
15.6 pg/mL-1000 pg/mL
Sensitivity
3.9 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Developmental Biology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse EDA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 84  
Range % 80-88  
1:2 Average % 91  
Range % 85-97  
1:4 Average % 90  
Range % 85-94  
1:8 Average % 102  
Range % 98-107  
Recovery
The recovery of mouse EDA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 89 85-93  
EDTA plasma (n=4) 95 90-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1000 2.383 2.253 2.318 2.161  
500 1.782 1.753 1.768 1.611  
250 1.097 1.145 1.121 0.964  
125 0.693 0.740 0.717 0.560  
62.5 0.450 0.461 0.456 0.299  
31.25 0.316 0.305 0.311 0.154  
15.6 0.251 0.242 0.247 0.090  
0 0.159 0.154 0.157    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse EDA ELISA Kit was designed for the quantitative measurement of Mouse EDA protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

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Target Background

Function
(From Uniprot)
Cytokine which is involved in epithelial-mesenchymal signaling during morphogenesis of ectodermal organs. Functions as a ligand activating the DEATH-domain containing receptors EDAR and EDA2R. Isoform TAA binds only to the receptor EDAR, while isoform TA-A2 binds exclusively to the receptor EDA2R. May also play a role in cell adhesion.; Isoform TAA binds only to the receptor EDAR, while isoform TA-A2 binds exclusively to the receptor EDA2R.; Isoform TA-A2 binds exclusively to the receptor EDA2R.
Gene References into Functions
  1. Eda initiates a signaling cascade and recruits a BAF complex to specific gene loci to facilitate transcription during organogenesis. PMID: 30037996
  2. Gain- and loss-of-function approaches reveal that liver-derived EDA regulates systemic glucose metabolism, suggesting that EDA is a hepatokine that can contribute to impaired skeletal muscle insulin sensitivity in obesity. PMID: 29106399
  3. This is the first analysis of the role of Eda in the root, showing a direct role for this pathway during postnatal mouse development, and it suggests that changes in proliferation and angle of HERS may underlie taurodontism in a range of syndromes. PMID: 28813629
  4. Data suggest that corneal epithelial integrity is defective and the thickness is reduced in Eda mutant Tabby mice at early postnatal stage (4-week-old); corneal epithelial cell proliferation is decreased and epithelial wound healing is delayed in Tabby mice, whereas it is restored by exogenous Eda. Tabby mice are a model for X-linked hypohidrotic ectodermal dysplasia and can be treated with recombinant Eda. PMID: 28655773
  5. Murine nasal submucosal glands express EDA during embryonic development. EDA signalling is essential for Lateral Nasal Gland and Medial Nasal Gland budding and ductal morphogenesis. PMID: 27590203
  6. Mouse models with HED also carry Eda, Edar or Edaradd mutations and have defects that map to the same structures.We report that otitis media, rhinitis and nasopharyngitis occur at high frequency in Eda and Edar mutant mice and explore the pathogenic mechanisms related to glandular function, microbial and immune parameters in these lines PMID: 27378689
  7. Wnt, Eda, and Shh have roles in touch dome Merkel cell development PMID: 27414798
  8. Using an ex vivo culture system, we show that suppression of canonical Wnt signalling leads to a dose-dependent inhibition of supernumerary placodes in K14-Eda tissue explants. PMID: 26581094
  9. Eda and activin A are upstream regulators of Foxi3 in skin appendage placodes PMID: 23441037
  10. Ectodysplasin regulates activator-inhibitor balance in murine tooth development through Fgf20 signaling. PMID: 22833125
  11. NF-kappaB downstream of the TNF-like ligand ectodysplasin (Eda) is identified as a unique regulator of embryonic and prepubertal ductal morphogenesis. PMID: 22451941
  12. Data found that Eda regulates growth and branching of the SMG via transcription factor NF-kappaB in the epithelium, and that the hedgehog pathway is an important mediator of Eda/NF-kappaB. PMID: 21652647
  13. Dkk4 affects an auxiliary pathway for Eda-independent development of secondary hair PMID: 20386733
  14. Treatment of Eda-/- minor salivary gland explants with EDA A1 rescues minor salivary gland induction. PMID: 20969842
  15. The ectodysplasin-A gene plays a role in normal bone development in mice. PMID: 11945012
  16. Eda and Edar expression is confined to the ectoderm and occurs in a pattern that suggests a role of ectodysplasin/Edar signaling in the interactions between the ectodermal compartments and the formation and function of hair placodes. PMID: 11973284
  17. Gene expression profiling data are consistent with the regulation of the NF-kappa B and JNK pathways by EDA PMID: 12095918
  18. ectodysplasin-Edar signaling has several roles in ectodermal organ development controlling their initiation, as well as morphogenesis and differentiation. PMID: 12812793
  19. Analysis of expression patterns of eda, edar and tnfrsf19 in mouse embryogenesis. PMID: 12972005
  20. Eda-A1 is among the key regulators of pattern formation in the skin PMID: 14502575
  21. Inducible Eda-A1 transgene mediates sebaceous gland hyperplasia and differential formation of two classes of mouse hair follicles. PMID: 14506134
  22. crystal structures of EDA-A1 and EDA-A2 show splice variants with distinct receptor specificity PMID: 14656435
  23. EDA-A2 transgenic mice exhibited multifocal myodegeneration; this phenotype was not observed in the absence of XEDAR PMID: 14749376
  24. Promotes placodal cell fate during early development of ectodermal organs. PMID: 15371307
  25. with increasing expression levels of this one gene, the number of cusps increases, cusp shapes and positions change, longitudinal crests form, and number of teeth increases PMID: 15538367
  26. isoforms of EDA-A5 and A5',activated NF-kappaB through receptors EDAR and XEDAR PMID: 16423472
  27. Data show that, as an ectodysplasin target, lymphotoxin-beta regulates hair phenotype in developing hair follicles. PMID: 16738056
  28. EDA signaling has a role in skin appendage development [review] PMID: 17102627
  29. Localization of Shh expression by Wnt and Eda affects axial polarity and shape of hairs. PMID: 17376426
  30. By analogy to the role played by eda in other ectodermal derivatives, we propose that it might play a role in defining the pattern of the palatal rugae. PMID: 18028050
  31. Microarray profiling of genes differentially regulated by short exposure to recombinant Eda-A1 in embryonic eda(-/-) skin explants, was performed to identify direct targets of ectodysplasin pathway. PMID: 18508042
  32. EDA signaling is needed for the determination and development of various skin appendages at spatiotemporally restricted intervals. PMID: 18923450
  33. The upper jaw is more sensitive than the lower jaw to the loss of eda function. Modifications in cusp number and the abnormal crown size of the teeth are clearly linked, and our results indicate a role of eda in cusp patterning. PMID: 19051250
  34. results indicate that unknown mechanisms of the Eda pathway are implicated in tooth morphogenesis PMID: 19340299
  35. Salivary gland branching morphogenesis: a quantitative systems analysis of the Eda protein PMID: 19500387
  36. The collagen domain activates EDA1 by multimerization, whereas the proteoglycan-binding domain may restrict the distribution of endogeneous EDA1 in vivo. PMID: 19657145

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Involvement in disease
Defects in Eda are the cause of the tabby phenotype in mice (the equivalent of anhidrotic ectodermal dysplasia in humans). The disease is characterized by sparse hair (atrichosis or hypotrichosis), abnormal or missing teeth and the inability to sweat due to the absence of sweat glands.
Subcellular Location
Cell membrane; Single-pass type II membrane protein.; [Ectodysplasin-A, secreted form]: Secreted.
Protein Families
Tumor necrosis factor family
Database Links
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