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Mouse Fibroblast growth factor 5(FGF5) ELISA kit

Citations (1) Protocol
Code CSB-EL008632MO
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
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Product Details

Target Name
fibroblast growth factor 5
Alternative Names
Fgf5 ELISA kit; Fgf-5Fibroblast growth factor 5 ELISA kit; FGF-5 ELISA kit; Heparin-binding growth factor 5 ELISA kit; HBGF-5 ELISA kit
Abbreviation
FGF5
Uniprot No.
P15656
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
31.25 pg/mL-2000 pg/mL
Sensitivity
7.8 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse FGF5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)
1:1 Average % 97
Range % 92-103
1:2 Average % 91
Range % 86-99
1:4 Average % 91
Range % 88-94
1:8 Average % 101
Range % 96-106
Recovery
The recovery of mouse FGF5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 90 87-94
EDTA plasma (n=4) 106 101-111
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2000 2.555 2.633 2.594 2.485
1000 2.142 2.258 2.200 2.091
500 1.567 1.614 1.591 1.482
250 1.033 1.102 1.068 0.959
125 0.725 0.754 0.740 0.631
62.5 0.471 0.445 0.458 0.349
31.25 0.214 0.221 0.218 0.109
0 0.105 0.112 0.109 　
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse FGF5 ELISA Kit was designed for the quantitative measurement of Mouse FGF5 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.

Citations

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Target Background

Function
(From Uniprot)
Plays an important role in the regulation of cell proliferation and cell differentiation. Required for normal regulation of the hair growth cycle. Functions as an inhibitor of hair elongation by promoting progression from anagen, the growth phase of the hair follicle, into catagen the apoptosis-induced regression phase.
Gene References into Functions
  1. Recruited CD11b+ CD68+ Kupffer cells/Mphis may play an essential role in steatohepatitis and fibrosis in FGF5 null mice fed with a HFD. Recruitment and activation of bone marrow derived Mphis is the key factor to develop steatohepatitis from simple steatosis. PMID: 27708340
  2. It participates in the progression of hepatic fibrosis. PMID: 24521867
  3. Interaction of mutant genes Fgf5(go-Y), we, and wal changes the duration of hair growth cycles in mice PMID: 22567929
  4. A deletion of a 9.3-kb region in the Fgf5 gene showed insertion of a 498-bp early transposon element long terminal repeat. Results show that long hair mutation of moja/moja mice is caused by Fgf5 disruption mediated by retrotransposon insertion. PMID: 21512271
  5. parathyroid hormone-related protein and fibroblast growth factor-5 regulate the anagen to catagen transition by independent pathways PMID: 12713572
  6. the Fgf5(go) gene is a powerful modifier of mutant genes determining the process of alopecia. PMID: 19534432

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Subcellular Location
Secreted.
Protein Families
Heparin-binding growth factors family
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Related pathways
MAPK signaling pathway PI3K-Akt signaling pathway Regulation of actin cytoskeleton Ras signaling pathway Rap1 signaling pathway
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