Mouse Interleukin-19(IL19) ELISA kit

Code CSB-EL011612MO
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
interleukin 19
Alternative Names
Il19Interleukin-19 ELISA Kit; IL-19 ELISA Kit
Abbreviation
IL19
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
12.5 pg/mL-800 pg/mL
Sensitivity
3.12 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL19 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 107    
Range % 96-112    
1:2 Average % 103    
Range % 94-107    
1:4 Average % 95    
Range % 86-99    
1:8 Average % 100    
Range % 90-104    
Recovery
The recovery of mouse IL19 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 102 88-106    
EDTA plasma (n=4) 103 92-107    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average Corrected    
800 2.707 2.656 2.682 2.542    
400 1.494 1.544 1.519 1.379    
200 0.829 0.845 0.837 0.697    
100 0.422 0.446 0.434 0.294    
50 0.309 0.313 0.311 0.171    
25 0.201 0.212 0.207 0.067    
12.5 0.176 0.171 0.174 0.034    
0 0.142 0.138 0.140      
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse IL19 ELISA Kit was designed for the quantitative measurement of Mouse IL19 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 pg/mL-800 pg/mL and the sensitivity is 3.12 pg/mL.

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Target Background

Function
(From Uniprot)
May play some important roles in inflammatory responses. Up-regulates IL-6 and TNF-alpha and induces apoptosis.
Gene References into Functions
  1. Data, including data from studies using knockout mice, suggest that interleukin-19 suppresses hapten-dependent skin inflammation in elicitation phase of contact hypersensitivity. PMID: 29386478
  2. Attenuated colitis in IL-19-deficient animals was associated with reduced numbers of IL-6-producing macrophages in the inflamed colonic lamina propria. Microbial-driven expression of IL-19 by intestinal macrophages may contribute to the pathogenesis of inflammatory bowel disease. PMID: 28864472
  3. IL-19 can promote angiogenesis in the absence of hypoxia by at least two distinct mechanisms: 1) direct effects on vascular cells and 2) indirect effects by stimulation of macrophages. PMID: 27053520
  4. IL-19 plays an anti-inflammatory role in the Th2-mediated colitis model. PMID: 27942772
  5. microglia may self-limit their inflammatory response by producing the negative regulator IL-19. PMID: 25794104
  6. Lack of IL-19 exacerbates intimal hyperplasia PMID: 24814101
  7. we delineate the detailed molecular pathway for IL-4 up-regulation of IL-19 in keratinocytes, which may play an important role in AD pathogenesis. PMID: 24943510
  8. Il-19 expression is increased in astrocytes following bacterial challenge. PMID: 24677051
  9. IL-19 is important for cutaneous wound healing because it upregulates KGF expression. PMID: 23582717
  10. IL-19 is a potent inhibitor of experimental atherosclerosis. PMID: 23950143
  11. IL-19 is pivotal in the pathogenesis of breast cancer. PMID: 22186257
  12. endogenous IL-19 is a constituent of the regulome that controls both processes in vivo PMID: 22110701
  13. IL-19 drives pathogenic innate immune responses in the colon. PMID: 19834971
  14. In a dust mite-induced murine model of asthma, the IL-19 level in asthmatic BALB/cJ mice is twice that of healthy control mice. PMID: 15557163

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Subcellular Location
Secreted.
Protein Families
IL-10 family
Database Links
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