Code | CSB-EL012719MO |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of mouse LAG3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:5 | Average % | 93 | ||||
Range % | 89-97 | |||||
1:10 | Average % | 104 | ||||
Range % | 98-107 | |||||
1:20 | Average % | 91 | ||||
Range % | 84-94 | |||||
1:40 | Average % | 99 | ||||
Range % | 94-102 |
The recovery of mouse LAG3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 90 | 85-95 | ||||
EDTA plasma (n=4) | 95 | 88-98 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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This mouse LAG3 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of mouse LAG3 in multiple samples, including serum, plasma, or tissue homogenates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the LGA3 levels in the sample and is measured at 450 nm through a microplate reader.
LAG3 is mainly expressed in activated T and natural killer (NK) cells and was identified as a marker for the activation of CD4+ and CD8+ T cells. Under pathological conditions, such as chronic inflammation or tumor environment, increased LAG3 expression on T cells was observed in combination with other inhibitory receptors such as PD-1, T cell immunoglobulin, and TIGIT, TIM3, CD160, 2B4, which finally led to T cell dysfunction. LAG3 is an important immune checkpoint with relevance in cancer, infectious disease, and autoimmunity. Furthermore, LAG3 was mainly found on tumor-infiltrating Tregs in many types of cancer when compared with non-malignant peripheral cells.
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