Code | CSB-E07272m |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ADP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
CUSABIO's mouse adiponectin (ADP) ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative determination of adiponectin concentrations in serum, plasma, cell culture supernates, tissue homogenates, and cell lysates. This assay exclusively recognizes mouse adiponectin. The quantitative sandwich ELISA technique of this kit is based on adiponectin antibody-adiponectin antigen interactions and an HRP colorimetric detection system to detect the levels of adiponectin in samples. The intensity of the color is positively proportional to the amount of bound adiponectin in the initial step.
Adiponectin, the most abundant plasma adipokine, plays an important role in the regulation of glucose, lipid metabolism, and insulin sensitivity through its anti-inflammatory, anti-fibrotic, anti-oxidant, angiogenic, and vasodilatory properties. Through its receptor AdipoR1 and AdipoR2, adiponectin signaling is involved in numerous essential regulatory cellular signaling mechanisms including AMPK, p38/MAPK signaling, STAT3, and lipid metabolic pathways. Weight loss or caloric restriction results in increasing adiponectin levels, and this increase is related to enhanced insulin sensitivity. Low plasma levels of adiponectin, also known as hypoadiponectinemia, are linked to the increased prevalence of obesity-associated diseases, including type 2 diabetes, coronary heart disease, and hypertension.
Sample type: Serum
Sample species: Mouse
Review: I used CSB-E07272m to detect the serum of C57 mice induced by high-fat diet, including normal diet, high-fat induced model group and high-fat induced administration group, and the dilution ratio was 1: 2000. Because the laboratory microplate reader is old, the dilution multiple should be 1: 4000, which may lead to better results. At the same time, the maximum concentration of 10ng/ml in the standard curve is similar to 5ng/ml, so the od value of the maximum concentration is discarded.
By Anonymous