Mouse adiponectin,ADP ELISA Kit

Code CSB-E07272m
Size 96T,5×96T,10×96T
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Product Details

Target Name
adiponectin, C1Q and collagen domain containing
Alternative Names
Adipoq ELISA Kit; Acdc ELISA Kit; Acrp30 ELISA Kit; Apm1 ELISA Kit; Adiponectin ELISA Kit; 30 kDa adipocyte complement-related protein ELISA Kit; Adipocyte complement-related 30 kDa protein ELISA Kit; ACRP30 ELISA Kit; Adipocyte ELISA Kit; C1q and collagen domain-containing protein ELISA Kit; Adipocyte-specific protein AdipoQ ELISA Kit
Abbreviation
ADP
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates, cell lysates
Detection Range
0.156 ng/mL-10 ng/mL
Sensitivity
0.039 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Sandwich
Precision

 

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

 

Linearity

 

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ADP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

 

Typical Data

 

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

CUSABIO's mouse adiponectin (ADP) ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative determination of adiponectin concentrations in serum, plasma, cell culture supernates, tissue homogenates, and cell lysates. This assay exclusively recognizes mouse adiponectin. The quantitative sandwich ELISA technique of this kit is based on adiponectin antibody-adiponectin antigen interactions and an HRP colorimetric detection system to detect the levels of adiponectin in samples. The intensity of the color is positively proportional to the amount of bound adiponectin in the initial step.

Adiponectin, the most abundant plasma adipokine, plays an important role in the regulation of glucose, lipid metabolism, and insulin sensitivity through its anti-inflammatory, anti-fibrotic, anti-oxidant, angiogenic, and vasodilatory properties. Through its receptor AdipoR1 and AdipoR2, adiponectin signaling is involved in numerous essential regulatory cellular signaling mechanisms including AMPK, p38/MAPK signaling, STAT3, and lipid metabolic pathways. Weight loss or caloric restriction results in increasing adiponectin levels, and this increase is related to enhanced insulin sensitivity. Low plasma levels of adiponectin, also known as hypoadiponectinemia, are linked to the increased prevalence of obesity-associated diseases, including type 2 diabetes, coronary heart disease, and hypertension.

Citations

Customer Reviews and Q&A

 Customer Reviews
Average Rating:
5.0 - 1 reviews

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Sample type: Serum

Sample species: Mouse

Review: I used CSB-E07272m to detect the serum of C57 mice induced by high-fat diet, including normal diet, high-fat induced model group and high-fat induced administration group, and the dilution ratio was 1: 2000. Because the laboratory microplate reader is old, the dilution multiple should be 1: 4000, which may lead to better results. At the same time, the maximum concentration of 10ng/ml in the standard curve is similar to 5ng/ml, so the od value of the maximum concentration is discarded.

By Anonymous

Target Background

Function
(From Uniprot)
Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
Gene References into Functions
  1. These data suggest that APN has a moderate regulatory role in oxidative stress-induced mitophagy and suppresses apoptosis. These findings demonstrate the antioxidant potential of APN in oxidative stress-associated skeletal muscle diseases. PMID: 28600493
  2. Downregulation of adiponectin in inflamed adipocyte by fetuin-A through the mediation of Wnt3a and PPARgamma is a new report. PMID: 27720679
  3. Study results indicate for the first time that adiponectin is able to influence the mechanical responses in strips from the mouse gastric fundus. PMID: 30254407
  4. miR-711, which is upregulated by Adipoq, represses TLR4 signaling, acting therefore as a major mediator of the anti-inflammatory action of Adipoq. PMID: 28240307
  5. both paracrine and endocrine effects of adiponectin may contribute to reduced reactive oxygen species generation and apoptosis after MI/R, in a CD36-dependent manner PMID: 29018142
  6. These data may indicate that insulin resistance in Adp(-/-) mice is likely caused by an increase in concentrations of TNFalpha and FFA via downregulation of PPARalpha. PMID: 29445073
  7. Adiponectin improves metabolic health but has only minor effects on reproductive functions in the polycystic ovary syndromemouse model. PMID: 28790184
  8. APN attenuates adverse cardiac remodeling following cardiac injury by up-regulating MMP-9 expression. APN up-regulates MMP-9 expression via activation of AMPK and ERK1/2. PMID: 29263115
  9. the effects of APN on the promotion of preadipocyte differentiation under inflammatory conditions may involve the PPARgamma signaling pathway, and at least partly depends on upregulation of PPARgamma expression. PMID: 29115433
  10. Results demonstrate that adiponectin enhances inhibitory postsynaptic current onto neuropeptide Y (NPY) neurons to attenuate action potential firing in NPY neurons in a glucose-independent manner, being contrasted to its glucose-dependent effect on proopiomelanocortin neurons. PMID: 28606559
  11. Results show a reciprocal regulation of adiponectin and FGF19 gene expression in mice. PMID: 27666676
  12. Acrp30 (a globular form of adiponectin) reduces the expression of proinflammatory cytokines and the expression of RAGE as beta amyloid transporters into brain. Moreover, Acrp30 attenuated the apoptosis and the tight junction disruption through AdipoR1-mediated NF-kappaB pathway in beta amyloid-exposed bEnd.3 cells. PMID: 29022894
  13. Findings demonstrate that adiponectin is an essential regulator of thermogenesis, and adiponectin is required for maintaining body temperature under cold exposure. PMID: 29058611
  14. Chronic stress accelerates DPP4-mediated GLP-1 degradation and alters plasma adiponectin, accelerating vascular senescence and impairing ischemia-induced neovascularization. PMID: 28963101
  15. AdipoQ antisense (AS) Long noncoding RNA (lncRNA) transfer from nucleus to cytoplasm inhibits adipogenesis through formation of an AdipoQ AS lncRNA/AdipoQ mRNA duplex to suppress the translation of AdipoQ mRNA.[ PMID: 29414510
  16. Adiponectin enhances quiescence exit of murine hematopoietic stem cells and hematopoietic recovery through mTORC1 potentiation. PMID: 28480607
  17. plasma adiponectin and leptin were also decreased in IL 10tm.These findings suggest that frailty observed in this mouse model of chronic inflammation may in part be driven by alterations in fat mass, hormone secretion and energy metabolism PMID: 29267271
  18. CpG ODNs decreased placental adiponectin expression in NOD mice and impaired human trophoblast function and was associated with increased embryo loss. Adiponectin may therefore play an important protective role in the prevention of bacteria-induced pregnancy failure. PMID: 27094728
  19. Caloric restriction (CR) impacted adiposity but only levels of the high molecular weight isoform of adiponectin responded to CR. PMID: 28156058
  20. T-cadherin was essential for accumulation of adiponectin in the neointima and atherosclerotic plaque lesions, and the adiponectin-T-cadherin association protected against vascular injury. PMID: 28062540
  21. mTORC1 mediated many of the beneficial actions of FGF21 in vitro, including UCP1 and FGF21 induction, increased adiponectin secretion, and enhanced glucose uptake without any adverse effects on insulin action. PMID: 27681418
  22. AnxA6 protein in adipocytes was upregulated by oxidative stress which might trigger AnxA6 induction in adipose tissues and contribute to impaired fat storage and adiponectin release. PMID: 27702590
  23. Adiponectin alters calcium and phosphate balance and renal mineral excretion, in part, through klotho. PMID: 27914707
  24. Adiponectin (ApN) proves to be a powerful protector of the skeletal muscle capable of reversing the disease progression, thus making it a potential therapeutic agent for Duchenne muscular dystrophy (DMD). PMID: 28463682
  25. These results demonstrated that LC along with insulin increases GSH levels thereby improving adiponectin secretion and glucose utilization in adipocytes. PMID: 28755973
  26. Adiponectin, TNF-alpha, and LOX-1 exert complex regulatory effects on the coronary microvascular endothelial function in atherosclerotic ApoE knockout mice. PMID: 27050429
  27. adiponectin inhibited endoplasmic reticulum stress and apoptosis of adipocyte in vivo and in vitro by activating the AMPK/PPARalpha/ATF2 pathway. PMID: 27882945
  28. Irisin improved endothelial function by modulating HO-1/ adiponectin axis in perivascular adipose tissue (PVAT) in HFD-induced obese mice. These findings suggest that regulating PVAT function may be a potential mechanism by which irisin improves endothelial function in obesity. PMID: 28595178
  29. a unique key feature of the T-cad prodomain is its involvement in binding of the T-cad repeats 1 and 2 to adiponectin; adiponectin positively regulates T-cad abundance PMID: 28325833
  30. adiponectin maintains intestinal homeostasis and protects against murine colitis through interactions with its receptor AdipoR1 and by modulating adaptive immunity and STAT3 signaling PMID: 28258220
  31. The KIF5B level was up-regulated during 3T3-L1 adipogenesis. This increase in cytosolic KIF5B was synchronized with the induction of adiponectin. Endogenous KIF5B and adiponectin were partially colocalized at the peri-nuclear and cytosolic regions. PMID: 27264953
  32. The elevation in circulating levels of adiponectin and Fgf15 led to normalized hepatic and serum levels of bile acids, limited hepatic accumulation of toxic bile, attenuated inflammation, and amelioration of liver injury in the ethanol-fed mNT knockout mice. PMID: 27573244
  33. Female adiponectin null mice displayed impaired fertility, reduced oocytes, disrupted estrous cycle, increased atretic follicles, and impaired late folliculogenesis. There was decrease in serum estradiol and FSH but an increase in LH and testosterone at proestrus. There was reduction of progesterone levels at diestrus, a significant decrease in LH receptor expression as well as in the number of GnRH immunoreactive neurons PMID: 27700136
  34. Tongqiaohuoxue decoction improved obesity-induced inflammation and insulin resistance by increasing adiponectin production. PMID: 27404230
  35. High salt is an important suppressor of cardioprotective APN and AdipoR1 in cardiac myocytes. PMID: 28051329
  36. PPARdelta activation in perirenal fat by agonist or high sodium intake inhibited renal sodium-glucose cotransporter 2 (SGLT2) function, which is mediated by increased production of adipose adiponectin. PMID: 27053360
  37. Acute knockdown of Insr or both Irs1 and Irs2 in adipocytes increased Adipoq mRNA expression but reduced adiponectin secretion. PMID: 26888756
  38. Adiponectin may protect the aorta from atherosclerotic injury by reducing inflammation. Adiponectin effectively inhibits the activation of NF-kappa B pathway by inhibiting the expression of NF-kappa B nuclear protein p65. PMID: 26965176
  39. Altered adiponectin multimerization could explain declined adiponectin levels and altered distribution of adiponectin complexes in the plasma of obese insulin-resistant individuals PMID: 26407855
  40. The expression of PI3K-insensitive GSK3 stimulates the production of adiponectin and protects from diet-induced metabolic syndrome. PMID: 27140617
  41. These data suggest that adiponectin could represent a possible biomarker in obesity-associated asthma PMID: 26462929
  42. ADPN might act as a biomarker of inflammation and have potential for the treatment of hemorrhagic shock. PMID: 26909947
  43. Adiponectin exerts novel effects to limit the production and action of mono-MPs, underscoring yet another pleiotropic effect of this adipokine. PMID: 26687997
  44. The results demonstrated a dynamic dysfunction of APN/AdipoR1 axis accompanying progression of diabetes mellitus in mice with cerebral ischemia. PMID: 26611106
  45. reduced plasma levels in obese mice treated with house dust mite allergens as compared to similarly treated lean mice PMID: 26476732
  46. CRP decreased adiponectin expression and multimerization, while CRP-induced decline in adiponectin might be mediated through the PI3K/Akt pathway. PMID: 26812237
  47. Globular Adiponectin (gAd) activates autophagy in myoblasts and gAd-activated autophagy drives the myogenic properties of this hormone. PMID: 26826036
  48. APN can help to reduce periodontitis-related bone loss, modulate JMJD3 and IRF4 expression, and macrophage infiltration. PMID: 26399931
  49. our data indicate that robust hypertrophic MEF2 activation in the heart in vivo requires a background of adiponectin signaling and that adiponectin signaling in primary isolated CM directly enhances MEF2 activity through activation of p38 MAPK PMID: 26196305
  50. In 3T3-L1 adipocytes, catechin and quercetin attenuated TNF-alpha-induced elevated protein carbonyls, increased proinflammatory cytokine expression (MCP-1, resistin), and decreased adiponectin. PMID: 25620282

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Subcellular Location
Secreted.
Tissue Specificity
Synthesized exclusively by adipocytes and secreted into plasma.
Database Links
CUSABIO guaranteed quality
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