Mouse coagulation factor Ⅴ,FⅤ ELISA Kit

Code CSB-E17815m
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
coagulation factor Ⅴ,FⅤ
Alternative Names
F5Coagulation factor V ELISA Kit; Activated protein C cofactor) [Cleaved into: Coagulation factor V heavy chain; Coagulation factor V light chain] ELISA Kit
Abbreviation
F5
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma
Detection Range
78 ng/mL-5000 ng/mL
Sensitivity
19.5 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Blood Coagulation
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse FⅤ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:100Average %96
Range %91-103
1:200Average %100
Range %95-104
1:400Average %95
Range %90-100
1:800Average %98
Range %94-105
Recovery
The recovery of mouse FⅤ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9185-96
EDTA plasma (n=4)8884-93
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
50002.351 2.521 2.436 2.240
25001.823 1.907 1.865 1.669
12501.354 1.294 1.324 1.128
6250.853 0.893 0.873 0.677
3120.612 0.607 0.610 0.414
1560.401 0.421 0.411 0.215
780.298 0.287 0.293 0.097
00.200 0.192 0.196
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse Coagulation factor V (F5) ELISA Kit was designed for the quantitative measurement of Mouse Coagulation factor V (F5) protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 78 ng/mL-5000 ng/mL and the sensitivity is 19.5 ng/mL.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
(From Uniprot)
Central regulator of hemostasis. It serves as a critical cofactor for the prothrombinase activity of factor Xa that results in the activation of prothrombin to thrombin.
Gene References into Functions
  1. Mice with the FVL mutation do not have increased spermatogenesis as compared to wildtype mice. PMID: 28927238
  2. Platelet-derived FV is a critical mediator of arterial thrombosis that involves platelet activation. PMID: 28673898
  3. Platelet-derived FV contributes to the control of angiogenesis and is likely associated with thrombin generation in hind limb ischemia model. PMID: 27237401
  4. Mice deficient in LMAN1 exhibit FV and FVIII deficiencies and liver accumulation of alpha1-antitrypsin. PMID: 21795745
  5. The FVL mutation does not influence coagulation activation, lung inflammation or survival in lethal influenza A. PMID: 20413539
  6. It suggested that there could be a combination of GLA deficiency and FVL or other thrombosis-related gene defect in patients with genetic severe early-onset thrombosis. PMID: 20533258
  7. that the murine platelet FV compartment is derived exclusively from primary biosynthesis within cells of marrow origin and an intact platelet FV pool is not required for protection from spontaneous hemorrhage or bleeding following minor trauma PMID: 12816857
  8. the murine platelet and plasma FV pools are biosynthetically distinct PMID: 12855561
  9. The source of the FVL leading to accelerated thrombosis appears to be circulating, non-platelet-derived plasma FVL. PMID: 15809370
  10. FVL has the ability to improve the hemophilia A or B phenotype, but this effect is principally evident at the microcirculation level following a particular vascular injury. PMID: 16359510
  11. observations demonstrate a synergistic interaction between alpha-galactosidase A deficiency and Factor V Leiden toward tissue fibrin deposition; concomitant mutations in these genes may increase the penetrance of vascular thrombotic events in humans PMID: 16514103
  12. Fetal gene defects precipitate platelet-mediated pregnancy failure in factor V Leiden mothers. PMID: 17438064
  13. The FVL mutation has no effect on the development of secondary tumours of colon cancer in livers of mice. PMID: 17635646
  14. FV Leiden allele has no effect in murine colitis and we thus question the importance of activated blood coagulation in the etiology or pathogenesis of inflammatory bowel disease. PMID: 17849058
  15. the factor V Leiden mutation is associated with enhanced thrombotic tendency after FeCl3 injury of the femoral artery on a standard fat diet, but not a high fat diet PMID: 17938812
  16. In contrast to humans, pregnancy induced a decrease in APC resistance in wild-type and in FV Leiden mice. PMID: 19036061
  17. Homozygous Factor V could promote atherosclerosis and fibrin deposition in apolipoprotein E deficient mice. PMID: 19671356

Show More

Hide All

Subcellular Location
Secreted.
Protein Families
Multicopper oxidase family
Database Links
CUSABIO guaranteed quality
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1
webinars: DT3C facilitates antibody internalization X