Code | CSB-E08326m |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of mouse iNOS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 96 | ||||
Range % | 90-102 | |||||
1:2 | Average % | 89 | ||||
Range % | 84-95 | |||||
1:4 | Average % | 102 | ||||
Range % | 98-107 | |||||
1:8 | Average % | 96 | ||||
Range % | 92-102 |
The recovery of mouse iNOS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 97 | 92-105 | ||||
EDTA plasma (n=4) | 95 | 91-99 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The mouse NOS2 ELISA Kit quantitates mouse NOS2 levels in serum, plasma, or tissue homogenates, cell lysates. iNOS, also called NOS2, is an enzyme that plays a vital role in the macrophage inflammatory response. Synthesis of NO by NOS2 is involved in crucial functions such as microbial killing and immune regulation and also is implicated in the pathophysiology of chronic myocardial remodeling and failure. NOS2 is expressed in vascular smooth muscle, endothelium, and myocardium in certain pathological conditions such as atherosclerosis, diabetes, and inflammatory disorders. Its expression is regulated by the IKKβ-NFκB pathway and mediates inflammation-involved insulin resistance.
This kit employs the sandwich-ELISA mechanism in conjugation with NOS2 antibody-NOS2 antigen-specific binding as well as HRP-TMB chromogenic reaction to measure the concentration of NOS2 in the samples. The kit is characterized by high sensitivity, strong specificity, good linearity, high recovery, and a precision of less than 10%.
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