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| Code | CSB-E08147m |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse TRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 90 | |||||
| Range % | 85-94 | ||||||
| 1:2 | Average % | 105 | |||||
| Range % | 91-113 | ||||||
| 1:4 | Average % | 94 | |||||
| Range % | 90-98 | ||||||
| 1:8 | Average % | 85 | |||||
| Range % | 80-89 | ||||||
| The recovery of mouse TRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 96 | 91-102 | |||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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This Mouse TRH ELISA Kit was designed for the quantitative measurement of Mouse TRH protein in serum. It is a Sandwich ELISA kit, its detection range is 2.5 pg/mL-160 pg/mL and the sensitivity is 0.625 pg/mL.
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I recently inquired about the TRH-ELISA kit through NacalaiTecque, Japan. I was informed that your TRH-ELISA kit only detects TRH and not pro-TRH. But this raises a new question: You provide Rat-TRH or human-TRH ELISA kits, how can these kits detect the differences between species? I don't think the TRH obtained as the final product varies from species to species.
We use different raw materials on the kits of different species. They have different characteristics as well as different matrix. As to the difference in detection results obtained from different species, we should take the result from the actual measurement as the real result.
