Pig Follicle-stimulating hormone,FSH ELISA Kit

Code CSB-E06791p
Size 96T,5×96T,10×96T
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Product Details

Target Name
Follicle-stimulating hormone,FSH
Alternative Names
FSHBFollitropin subunit beta ELISA Kit; Follicle-stimulating hormone beta subunit ELISA Kit; FSH-B ELISA Kit; FSH-beta ELISA Kit; Follitropin beta chain ELISA Kit
Abbreviation
FSH
Uniprot No.
Species
Sus scrofa (Pig)
Sample Types
serum, plasma, tissue homogenates
Detection Range
20 mIU/mL-700 mIU/mL
Sensitivity
10 mIU/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig FSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 90  
Range % 85-94  
1:2 Average % 94  
Range % 90-98  
1:4 Average % 92  
Range % 87-97  
1:8 Average % 84  
Range % 80-88  
Recovery
The recovery of pig FSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 90-99  
EDTA plasma (n=4) 97 91-101  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mIU/ml OD1 OD2 Average Corrected  
700 1.389 1.416 1.403 1.367  
250 0.578 0.615 0.597 0.561  
100 0.317 0.302 0.310 0.274  
50 0.163 0.154 0.159 0.123  
20 0.095 0.101 0.098 0.062  
0 0.037 0.034 0.036    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Pig FSH ELISA Kit was designed for the quantitative measurement of Pig FSH protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 20 mIU/mL-700 mIU/mL and the sensitivity is 10 mIU/mL.

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Target Background

Function
(From Uniprot)
Together with the alpha chain CGA constitutes follitropin, the follicle-stimulating hormone, and provides its biological specificity to the hormone heterodimer. Binds FSHR, a G protein-coupled receptor, on target cells to activate downstream signaling pathways. Follitropin is involved in follicle development and spermatogenesis in reproductive organs.
Gene References into Functions
  1. As in vitro experiments in the present study suggested that LHX2 regulated the expression of Fshbeta, it is possible that LHX2 controls FSH synthesis at the transcriptional level. PMID: 22134063
  2. Results showed that the effects of FSHb, ESR, and PRLR genes were significant in the Tibet pig population, and the effective genotypes of the three genes for reproductive traits were BB, BB, and AA, respectively. PMID: 20466637
  3. LHX3 LIM homeodomain transcription factor is involved in activation of the FSH beta-subunit gene in the pituitary gonadotrope cell. PMID: 15271874
  4. Prop-1 participates in the regulation of FSHbeta gene PMID: 15474519
  5. A allele of FSHbeta-1 locus in all the population and FSHbeta-2 locus in pigs of the first parity had the positive effective on TNB, NBA, and WB. PMID: 18065386

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Subcellular Location
Secreted.
Protein Families
Glycoprotein hormones subunit beta family
Database Links
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