Pig Immunoglobulin A,IgA ELISA Kit

Code CSB-E13234p
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
Immunoglobulin A,IgA
Abbreviation
IgA
Species
Sus scrofa (Pig)
Sample Types
serum, plasma
Detection Range
0.146 μg/mL-37.5 μg/mL
Sensitivity
0.146 μg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig IgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1000 Average % 92  
Range % 88-94  
1:2000 Average % 93  
Range % 90-98  
1:4000 Average % 88  
Range % 85-94  
1:8000 Average % 95  
Range % 91-100  
Recovery
The recovery of pig IgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 89-97  
EDTA plasma (n=4) 103 98-106  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/ml OD1 OD2 Average    
37.5 0.199 0.209 0.204    
9.375 0.445 0.420 0.433    
2.345 0.924 0.955 0.940    
0.586 1.413 1.447 1.430    
0.146 2.101 2.114 2.108    
0 2.547 2.503 2.525    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Pig IgA ELISA Kit was designed for the quantitative measurement of Pig IgA protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 0.146 μg/mL-37.5 μg/mL and the sensitivity is 0.146 μg/mL.

Citations

Customer Reviews and Q&A

 Customer Reviews
Average Rating:
5.0 - 2 reviews

Submit a Review here

Sample type: Serum

Sample species: Pig

Sample dilution: No dilution

Review: The calibration is accurate, and the sample values are within the calibration range, which is in line with the expected results.

By Anonymous

Sample type: Serum

Sample species: Pig

Sample dilution: No dilution

Review: The calibration is accurate, and the sample values are within the calibration range, which is in line with the expected results.

By Anonymous

 Q&A
Q:

Hi. I would like to know if this kit is used feces samples from pig. If so, I would like knowing the cost. Also I would like to know if you have avilable the kit with reagents for plate preparation and its respective cost, as well. Thanks much.

A:
Thanks for your inquiry.
For IgA, there are serotype and secretory type. If you want to test feces samples, we recommend you choose Pig secretory immunoglobulin A,SIgA ELISA KitCSB-E12063p. No matter you use CSB-E12063p or CSB-E13234p, we suggest you do a pretest first.
All the components in the kit are well prepared, so you don't need to prepare the reagents by yourself. Sorry we can not provide reagents for plate preparation.Pls let me know if you have any further questions. Thank you.
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