Pig terminal complement complex C5b-9 (TCC C5b-9)ELISA Kit

Code CSB-E15837p
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
terminal complement complex C5b-9 (TCC C5b-9)
Abbreviation
TCC C5b-9
Species
Sus scrofa (Pig)
Sample Types
serum, plasma, tissue homogenates
Detection Range
18.75 ng/mL-1200 ng/mL
Sensitivity
4.69 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig TCC C5b-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %99
Range %85-105
1:2Average %95
Range %85-113
1:4Average %95
Range %82-102
1:8Average %97
Range %85-110
Recovery
The recovery of pig TCC C5b-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9688-109
EDTA plasma (n=4)9785-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
12002.217 2.319 2.268 2.130
6001.790 1.767 1.779 1.641
3001.123 1.132 1.128 0.990
1500.675 0.651 0.663 0.525
750.397 0.403 0.400 0.262
37.50.285 0.298 0.292 0.154
18.750.231 0.216 0.224 0.086
00.141 0.134 0.138  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Pig TCC C5b-9 ELISA Kit was designed for the quantitative measurement of Pig TCC C5b-9 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 18.75 ng/mL-1200 ng/mL and the sensitivity is 4.69 ng/mL.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

 Q&A
Q:

At what wavelength do i read the results?

A:
Thanks for your inquiry.
CSB-E15837p
The detection wavelength must be 450nm.
The absorbance value will be very good at this wavelength. Considering there may be scratches at the bottom of the vial when you add samples, we suggest you use dual wavelength( detection wavelength and correction wavelength).
Pls let me know if you have any further questions. Thank you.
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1