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| Code | CSB-E13995p |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of pig TAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 84-100 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-108 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 82-105 | |||||
| The recovery of pig TAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 85-102 | ||||
| EDTA plasma (n=4) | 98 | 92-106 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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This Pig TAT ELISA Kit was designed for the quantitative measurement of Pig TAT protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.
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We are considering purchasing this product CSB-E13995p and I was reviewing the online documentation. The protocol specifies drawing blood samples using Li-heparin or K-EDTA as an anticoagulant. My laboratory has always used Na-citrate for ex-vivo anticoagulation when we are interested in measuring indices of coagulation like TAT. Has this variable been tested by CusaBio and is there a reason for what is put in the documentation?
For the detection of plasma, usually we use EDTA and heparin as anticoagulants. The Na-citrate mentioned by you has little effect in theory. Pls follow the actual test results.
