Rabbit Sex hormone-binding globulin(SHBG) ELISA kit

Code CSB-EL021252RB
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
sex hormone-binding globulin
Alternative Names
SHBG ELISA Kit; Sex hormone-binding globulin ELISA Kit; SHBG ELISA Kit; Sex steroid-binding protein ELISA Kit; SBP ELISA Kit; Testis-specific androgen-binding protein ELISA Kit; ABP ELISA Kit
Abbreviation
SHBG
Uniprot No.
Species
Oryctolagus cuniculus (Rabbit)
Sample Types
serum, plasma, tissue homogenates
Detection Range
25 pmol/mL-400 pmol/mL
Sensitivity
25 pmol/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit SHBG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 92
Range % 90-103
1:2 Average % 89
Range % 83-99
1:4 Average % 95
Range % 90-101
1:8 Average % 89
Range % 85-100
Recovery
The recovery of rabbit SHBG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 87-100
EDTA plasma (n=4) 95 89-101
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pmol/ml OD1 OD2 Average  
400 0.266 0.252 0.259  
200 0.528 0.541 0.535  
100 0.874 0.863 0.869  
50 1.348 1.325 1.337  
25 2.511 2.552 2.532  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rabbit SHBG ELISA Kit was designed for the quantitative measurement of Rabbit SHBG protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 25 pmol/mL-400 pmol/mL and the sensitivity is 25 pmol/mL.

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Target Background

Function
(From Uniprot)
Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration.
Subcellular Location
Secreted.
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