Rat 5'-nucleotidase/cluster of differentiation 73 (NT5E/CD73) ELISA kit

Code CSB-E14100r
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
5'-nucleotidase/cluster of differentiation 73 (NT5E/CD73)
Alternative Names
Nt5e ELISA kit; Nt5 ELISA kit; Nte5'-nucleotidase ELISA kit; 5'-NT ELISA kit; EC 3.1.3.5 ELISA kit; Ecto-5'-nucleotidase ELISA kit; CD antigen CD73 ELISA kit
Abbreviation
NT5E/CD73
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates, cell culture supernates
Detection Range
0.625 mIU/mL-40 mIU/mL
Sensitivity
0.156 mIU/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat CD73 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %90
Range %87-96
1:2Average %87
Range %82-95
1:4Average %90
Range %85-97
1:8Average %93
Range %86-101
Recovery
The recovery of rat CD73 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9590-101
EDTA plasma (n=4)9186-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mIU/mlOD1OD2AverageCorrected
402.504 2.416 2.460 2.358
201.933 1.824 1.879 1.777
101.386 1.283 1.335 1.233
50.814 0.749 0.782 0.680
2.50.424 0.397 0.411 0.309
1.250.228 0.213 0.221 0.119
0.6250.154 0.145 0.150 0.048
00.103 0.101 0.102  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

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Target Background

Function
(From Uniprot)
Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
Subcellular Location
Cell membrane; Lipid-anchor, GPI-anchor.
Protein Families
5'-nucleotidase family
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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